Stereotaxic approach to hypothalamic nuclei of the Shiba goat with radiographic monitoring.
Stereotaxic approach to hypothalamic nuclei of the Shiba goat with radiographic monitoring.
复制标题
通过放射线监测对柴山羊下丘脑核进行立体定位。
DOI：
10.1292/jvms1939.52.339
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Hoshino
中科院分区：
文献类型：
--
作者：
Y. Mori;Y. Takeuchi;M. Shimada;S. Hayashi;K. Hoshino
Practical method was devised for precise approach to hypothalamic nuclei in the Shiba goat. A stereotaxic instrument and a brain atlas with stereotaxic coordinates were developed. For an accurate placement of probes into specific hypothalamic regions a radiographic method was employed in which radio-opaque material was injected into the lateral ventricle and the ventricular outline was depicted. A sagittal diagram showing the arrangement of hypothalamic nuclei in relation to the brain ventricular system was constructed from the transverse stereotaxic atlas. This diagram was revealed extremely useful in pinpointing the target on the radiographs of lateral view. Precision of this method was evaluated in female Shiba goats (n = 4) by comparing radiographically estimated positions of hypothalamic nuclei with those histologically determined. Despite of cranial variability among individual animals these two parameters matched well each other in all the nuclei examined. Furthermore, chronic cannulae were implanted into different hypothalamic structures of one goat and the accuracy of their placement was confirmed histologically. Thus, it was revealed that the stereotaxy by aid of radiography herein described was accurate enough to apply to various neuroendocrinological studies in the Shiba goat.
