CAMP FACTOR OF GROUP-B STREPTOCOCCI - PRODUCTION, ASSAY, AND NEUTRALIZATION BY SERA FROM IMMUNIZED RABBITS AND EXPERIMENTALLY INFECTED COWS
CAMP FACTOR OF GROUP-B STREPTOCOCCI - PRODUCTION, ASSAY, AND NEUTRALIZATION BY SERA FROM IMMUNIZED RABBITS AND EXPERIMENTALLY INFECTED COWS
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DOI：
10.1128/iai.9.2.377-383.1974
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发表时间：
1974-01-01
影响因子：
3.1
通讯作者：
JOHNSON, DW
中科院分区：
文献类型：
--
作者：
BROWN, J;FARNSWORTH, R;JOHNSON, DW
描述了B组链球菌的CAMP因子活性的定量测定方法和动物血清中和该活性的方法。CAMP反应的过程受红细胞与葡萄球菌β-溶血素预孵育的影响，反应速率受CAMP因子浓度的影响。用麦芽糖代替发酵液中的葡萄糖可提高CAMP因子的产量。注射B群链球菌无菌培养物上清液的家兔血清中对cAMP活性的抑制滴度升高，表明家兔体内的cAMP因子是一种抗原。患有实验诱导的B组链球菌性乳腺炎的奶牛也表现出CAMP活性的中和滴度显著升高。
Quantitative assay methods for CAMP factor activity of group B streptococci and for neutralization of this activity by animal sera are described. The course of the CAMP reaction is influenced by preincubation of red cells with staphylococcal beta-hemolysin and the rate of the reaction by the concentration of CAMP factor. The production of CAMP factor is enhanced by substitution of maltose for glucose in culture broth. Rising inhibitory titers to CAMP activity in sera of rabbits injected with sterile culture supernates of group B streptococci suggest that CAMP factor is an antigen in the rabbit. Cows with experimentally induced group B streptococcal mastitis also demonstrated significant rises in neutralizing titers for CAMP activity.