DNA Nanotechnology-Empowered Live Cell Measurements.
DNA Nanotechnology-Empowered Live Cell Measurements.
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DOI：
10.1002/smll.202204711
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发表时间：
2022-09
期刊：
影响因子：
13.3
通讯作者：
Jiangbo Liu;Min Li;Xiaolei Zuo
中科院分区：
文献类型：
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作者：
Jiangbo Liu;Min Li;Xiaolei Zuo
对各种生物分子的系统分析和精确操作将导致基础生物学中前所未有的发现。然而，传统的技术已不能满足当前的要求。尽管如此，在过去的四十年里，随着DNA纳米技术发展到产生DNA纳米结构和电路，已经取得了进展。由于DNA的生物相容性、纳米可寻址性和刺激响应性，DNA纳米技术在活细胞测量中的许多潜在应用已经开始出现。本文综述了目前可用的DNA纳米技术支持的活细胞测量方法。首先总结了DNA纳米结构在细胞微环境中的稳定性，这对于完成精确的活细胞测量至关重要。然后，介绍了活细胞中细胞外和细胞内微环境的测量。最后，讨论了这一新兴领域所固有的挑战以及可能的进一步发展。
The systematic analysis and precise manipulation of a variety of biomolecules should lead to unprecedented findings in fundamental biology. However, conventional technology cannot meet the current requirements. Despite this, there has been progress as DNA nanotechnology has evolved to generate DNA nanostructures and circuits over the past four decades. Many potential applications of DNA nanotechnology for live cell measurements have begun to emerge owing to the biocompatibility, nanometer addressability, and stimulus responsiveness of DNA. In this review, the DNA nanotechnology-empowered live cell measurements which are currently available are summarized. The stability of the DNA nanostructures, in a cellular microenvironment, which is crucial for accomplishing precise live cell measurements, is first summarized. Thereafter, measurements in the extracellular and intracellular microenvironment, in live cells, are introduced. Finally, the challenges that are innate to, and the further developments that are possible in this nascent field are discussed.