Opposing activities of dally-like glypican at high and low levels of wingless morphogen activity
Opposing activities of dally-like glypican at high and low levels of wingless morphogen activity
复制标题
DOI：
10.1016/j.devcel.2004.08.005
复制
发表时间：
2004-10-01
影响因子：
11.8
通讯作者：
Cohen, SM
中科院分区：
文献类型：
--
作者：
Kreuger, J;Perez, L;Cohen, SM
硫酸肝素蛋白多糖的糖化家族与形态梯度的形成有关。在这里，我们研究了glyypican Dally-like protein （Dip）在果蝇翅盘无翼梯度形成中的作用。令人惊讶的是，我们发现Dip有相反的效果在高和低水平的无翼。Dip促进低级无翼活动，但减少高级无翼活动。我们提出的证据表明，Wg拮抗剂Notum在其GPI锚定水平上诱导Dip型蛋白的裂解，从而导致Dip的脱落。因此，Notum对Dip的空间调控修饰利用Dip的配体结合活性以上下文依赖的方式促进或抑制信号传导。notum诱导的Dip脱落可以将Dip从膜系固的辅助受体转化为分泌的拮抗剂。
The glypican family of heparan sulfate proteoglycans has been implicated in formation of morphogen gradients. Here, we examine the role of the glypican Dally-like protein (Dip) in shaping the Wingless gradient in the Drosophila wing disc. Surprisingly, we find that Dip has opposite effects at high and low levels of Wingless. Dip promotes low-level Wingless activity but reduces high-level Wingless activity. We present evidence that the Wg antagonist Notum acts to induce cleavage of the Dip glypican at the level of its GPI anchor, which leads to shedding of Dip. Thus, spatially regulated modification of Dip by Notum employs the ligand binding activity of Dip to promote or inhibit Signaling in a context-dependent manner. Notum-induced shedding of Dip could convert Dip from a membrane-tethered coreceptor to a secreted antagonist.