Lactoferrin modified doxorubicin-loaded procationic liposomes for the treatment of gliomas
Lactoferrin modified doxorubicin-loaded procationic liposomes for the treatment of gliomas
复制标题
乳铁蛋白修饰的负载阿霉素的前阳离子脂质体用于治疗神经胶质瘤
DOI：
10.1016/j.ejps.2011.07.007
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发表时间：
2011-09-18
影响因子：
4.6
通讯作者：
He, Qin
中科院分区：
文献类型：
--
作者：
Chen, Huali;Qin, Yao;He, Qin
In this study, a brain-targeted chemotherapeutical delivery system, doxorubicin-loaded lactoferrin-modified procationic liposome (DOX-Lf-PCL) was developed, and its therapeutic effect for glioma was evaluated. The uptake profile of various DOX formulations in vitro by primary brain capillary endothelial cells (BCECs) and glioma cell C6 were studied by laser scanning confocal microscope and flow cytometry. An intracranial tumor model of rats was employed to evaluate the therapeutic effect of DOX-Lf-PCLs for glioma. Five groups of glioma-bearing rats (total n = 50) were subjected to three cycles of 2.5 mg/kg body weight of doxorubicin in different formulations or normal saline (N.S.) and analyzed for survival (median survival time, Kaplan-Meier). The results indicated that compared with the DOX solution or DOX-loaded conventional liposomes (DOX-Lips). DOX-PCLs and DOX-Lf-PCLs showed an improved performance in the uptake efficiency in BCECs and C6 cells. The DOX-Lf-PCLs can inhibit the growth of C6 more efficiently in vitro than other DOX formulations. The endocytosis involved in the DOX-Lf-PCLs uptake of C6 was mediated by both receptor- and absorption-mediated transcytosis. DOX-Lf-PCLs could significantly extend the survival time compared with the N.S. control and other DOX formulations. This study showed that the therapy with DOX-Lf-PCLs offers an effective therapeutic potential for gliomas. (C) 2011 Elsevier B.V. All rights reserved.
