Alginate gel cell microspheres combined with biphasic calcium phosphate ceramics to form a dual 3D programmed culture system for bone regeneration
Alginate gel cell microspheres combined with biphasic calcium phosphate ceramics to form a dual 3D programmed culture system for bone regeneration
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海藻酸盐凝胶细胞微球与双相磷酸钙陶瓷结合形成双3D程序化培养系统用于骨再生
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通讯作者：
Bo Li
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作者：
Sheng Lian;Long Chen;Gong Zhang;Kun Peng;Bo Li
细胞活力低、细胞存活率低以及血管化能力不理想一直是组织工程骨应用面临的巨大挑战。构建三维培养系统模拟种子细胞的微环境可能是提高组织工程骨细胞活力、细胞存活率和血管化能力的潜在方法。因此，我们在前期工作的基础上，建立了一个双三维程序化骨再生培养系统。将干细胞藻酸盐珠接种到双相磷酸钙陶瓷中，形成双重3D程序化培养系统。根据成骨和血管生成相关生长因子的表达水平调整培养基和生长因子的含量。通过体外和体内实验对该双三维培养体系进行了评价。结果表明，藻酸盐凝胶细胞微球与BCPCs联合应用能更有效地促进骨和血管的再生。本研究为该双三维培养系统的临床应用提供了新的实验依据和理论依据。
Low cell viability, cell survival rate and unsatisfactory vascularization ability have always been a great challenge for the application of tissue engineered bone. Building a 3D culture system to simulate the micro-environment of seed cells may be a potential method to improve the cell viability, cell survival rate and vascularization ability of tiusse engineered bone. Thus, based on our previous work, we aimed to build a dual 3D programmed culture system for bone regeneration. Stem cell alginate beads were seeded into biphasic calcium phosphate ceramics to form a dual 3D programmed culture system. The content of the culture medium and the growth factors were adjusted based on the expression levels of osteogenesis and angiogenesis related growth factors. This dual 3D culture system was estimated by in vitro and in vivo experiment. The results indicated that alginate gel cell microspheres combined with BCPCs stimulated the regeneration of bone and vessels more effectively than other groups. Our research provides new experimental evidence and a theoretical basis for clinical application of this dual 3D culture system.