MicroRNA-21 (miR-21) Post-Transcriptionally Downregulates Tumor Suppressor PDCD4 and Promotes Cell Transformation, Proliferation, and Metastasis in Renal Cell Carcinoma
MicroRNA-21 (miR-21) Post-Transcriptionally Downregulates Tumor Suppressor PDCD4 and Promotes Cell Transformation, Proliferation, and Metastasis in Renal Cell Carcinoma
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DOI：
10.1159/000362946
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Song, Xishuang
中科院分区：
文献类型：
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作者：
Li, Xiancheng;Xin, Shiyong;Song, Xishuang
目的：MiR-21 诱导肿瘤转化、细胞增殖和转移，并下调某些癌症中的程序性细胞死亡 4 (PDCD4)。本研究的目的是探讨 PDCD4 和 miR-21 在人肾细胞癌 (RCC) 中的作用和相互作用。材料和方法：研究了来自 RCC 患者的总共 32 对肿瘤和正常组织标本以及三种肾癌细胞系（786-O、A498、caki-1）和一种正常上皮肾细胞系（HK-2）。通过蛋白质印迹分析、qRT-PCR 和荧光素酶报告基因测定来检查 PDCD4（蛋白质和 mRNA）和 miR-21 的表达水平。此外，我们用pre-miR-21（模拟物）和anti-miR-21（抑制剂）转染786-O细胞，然后再次分析PDCD4蛋白和mRNA的表达，并通过EDU和软琼脂集落形成实验测定细胞增殖和转化能力。结果：与正常非转移性 RCC 标本和 HK-2 细胞相比，MiR-21 表达在 RCC、转移性 RCC 标本和肾癌细胞系（A498、786-O、caki-1）中显着上调（P
Objectives: MiR-21 induces neoplastic transformation, cell proliferation, and metastasis and downregulates programmed cell death4 (PDCD4) in some cancers. The aim of this study was to investigate the roles and interactions of PDCD4 and miR-21 in human renal cell carcinoma (RCC). Materials and Methods: A total of 32 paired tumor and normal tissue specimens from RCC patients as well as three renal cancer cell lines (786-O, A498, caki-1) and one normal epithelial kidney cell line (HK-2) were studied. The expression levels of PDCD4 (protein and mRNA) and miR-21 were examined by Western blot analysis and by qRT-PCR and luciferase reporter assays. Furthermore, we transfected 786-O cells with pre-miR-21 (mimics) and anti-miR-21 (inhibitor) and then again analyzed the expression of PDCD4 protein and mRNA, and determined cell proliferation and transformation capabilities by EDU and soft agar colony formation assay. Results: MiR-21 expression was significantly upregulated in RCC, metastatic RCC specimens and renal cancer cell lines (A498, 786-O, caki-1) compared to normal non-metastatic RCC specimens and HK-2 cells (P