Downregulation of CD36 results in reduced phagocytic ability of peritoneal macrophages of women with endometriosis
Downregulation of CD36 results in reduced phagocytic ability of peritoneal macrophages of women with endometriosis
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DOI：
10.1002/path.2588
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发表时间：
2009-10-01
影响因子：
7.3
通讯作者：
Tsai, Shaw-Jenq
中科院分区：
文献类型：
--
作者：
Chuang, Pei-Chin;Wu, Meng-Hsing;Tsai, Shaw-Jenq
子宫内膜异位症被定义为子宫内膜组织在子宫腔外生长，是一种严重而复杂的疾病，影响超过 10% 的女性。子宫内膜异位症的病因尚不清楚，但免疫功能障碍可能是其发生的一个重要因素。免疫系统的自然功能是检测并破坏异常或不正常的细胞。免疫系统未能根除这些异常细胞通常会导致疾病发病机制。我们在此报告，从患有子宫内膜异位症的女性中分离的腹膜巨噬细胞中，巨噬细胞的吞噬能力降低。深入研究发现，子宫内膜异位症女性腹腔巨噬细胞中B类清道夫受体CD36的水平低于正常巨噬细胞。通过中和抗体阻断 CD36 功能或使用 siRNA 敲低 CD36 会损害正常巨噬细胞的吞噬能力。相比之下，从子宫内膜异位症女性体内分离出的巨噬细胞中强制表达 CD36 可恢复吞噬能力。综上所述，我们发现子宫内膜异位症女性腹腔巨噬细胞中清道夫受体CD36减少，导致巨噬细胞吞噬能力下降。这些发现揭示了子宫内膜异位症发展过程中免疫功能障碍的潜在机制。版权所有 (C) 2009 大不列颠及爱尔兰病理学会。由约翰·威利父子有限公司出版
Endometriosis, defined as the growth of endometrial tissues outside of the uterine cavity, is a severe and complex disease affecting more than 10% of women. The aetiology of endometriosis is unclear but immune dysfunction might be an important factor for its development. The natural function of the immune system is to detect and destroy aberrant or abnormal cells. Failure of the immune system to eradicate these aberrant cells often results in disease pathogenesis. We report here that the phagocytic ability of macrophages is reduced in peritoneal macrophages isolated from women with endometriosis. In-depth investigation revealed that the level of CD36, a class B scavenger receptor, in peritoneal macrophages derived from women with endometriosis was lower than that in normal macrophages. Blockage of CD36 function by neutralized antibody or knocking down CD36 using siRNA impaired the phagocytic ability of normal macrophages. In contrast, forced expression of CD36 in macrophages isolated from women with endometriosis restored phagocytic ability. Taken together, we identified that the scavenger receptor CD36 is reduced in the peritoneal macrophages of women with endometriosis, which leads to a decrease of the phagocytic ability of macrophages. These findings revealed a potential mechanism of immune dysfunction during endometriosis development. Copyright (C) 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.