Triphenyltin disrupts intracellular Zn2+ homeostasis in rat thymic lymphocytes
Triphenyltin disrupts intracellular Zn2+ homeostasis in rat thymic lymphocytes
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三苯基锡破坏大鼠胸腺淋巴细胞的细胞内 Zn2 稳态
DOI：
10.1016/j.tiv.2020.104782
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发表时间：
2020
影响因子：
3.2
通讯作者：
Oyama Yasuo
中科院分区：
文献类型：
--
作者：
Ueno Toshiya;Oyama Keisuke;Hyung Youn Jae;Ueno Shinya;Oyama Yasuo
三苯基锡（TPT）以前被用作农业杀菌剂和工业防污剂，现在被认为是一种环境污染物。TPT对人类健康的影响是令人担忧的，因为它是海产品中的一种污染物。本研究测定了氯化三苯基锡（TPTCH）诱导的大鼠胸腺淋巴细胞内Zn 2+浓度（[Zn 2 +] i）和细胞内非蛋白硫醇含量（[NPT] i）的变化。使用荧光探针FluoZin-3-AM和5-氯甲基荧光素二乙酸酯（5-CMF-DA）通过流式细胞术对此进行研究。与0.1 μ M或更高浓度（高达3 μ M）的TPTCH孵育，以浓度依赖性方式增加[Zn2 +] i。TPTCH诱导的[Zn~（2+）] i升高是由于膜Zn~（2+）通透性增加和细胞内Zn~（2+）释放增加。与0.3 μ M TPTCH孵育显著增加[NPT] i水平，而加入细胞内Zn 2+螯合剂对其无影响。较高浓度（1或3 μ M）的TPT降低[NPT] i. TPT可干扰淋巴细胞中的细胞内Zn 2+信号传导，从而干扰细胞功能。
Triphenyltin (TPT), previously used as an agricultural fungicide and industrial antifoulant, is now considered an environmental pollutant. The effect of TPT on human health is concerning due to its presence as a contaminant in seafood. In this study, the changes in intracellular Zn2+concentration ([Zn2+]i) and cellular content of nonprotein thiols ([NPT]i) induced by triphenyltin chloride (TPTCH), were measured in rat thymic lymphocytes. This was studied by flow-cytometry using the fluorescent probes FluoZin-3-AM and 5-chloromethylfluorescein diacetate (5-CMF-DA). Incubation with TPTCH, at 0.1 μM or more (up to 3 μM), increased [Zn2+]i in a concentration-dependent manner. The TPTCH-induced elevation in [Zn2+]i was due to the increase in membrane Zn2+permeability and intracellular Zn2+release. Incubation with TPTCH at 0.3 μM significantly increased [NPT]i levels, whereas the addition of an intracellular Zn2+chelator had no effect on the same. TPT at higher concentrations (1 or 3 μM) reduced [NPT]i. TPT may disturb intracellular Zn2+signaling in lymphocytes that disturbs cellular functions.