Control of specific gene expression by gibberellin and brassinosteroid
Control of specific gene expression by gibberellin and brassinosteroid
复制标题
DOI：
10.1104/pp.010173
复制
发表时间：
2001-10-01
期刊：
影响因子：
7.4
通讯作者：
Mundy, J
中科院分区：
文献类型：
--
作者：
Bouquin, T;Meier, C;Mundy, J
我们发现了一个隐性的，由油菜素内酯受体BRI1的缺失等位基因（BRI1 -201）引起的油菜素内酯不敏感突变。bri1-201突变体显示赤霉素（GA）差异调控基因的表达水平改变。RNA-blot分析显示，BR和GA可拮抗GA应答基因GASA1和GA抑制基因GA5 mrna的积累。环己亚胺表达研究表明，GA和BR对GA5的拮抗作用需要从头合成蛋白。报告基因分析和RNA-blot分析表明，BR和GA在转录水平上至少部分地调节GA5的表达，并且信号是相互独立和相减的。
We identified a recessive, brassinolide-insensitive mutant caused by a deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 mutant displayed altered expression levels of genes differentially regulated by gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate the accumulation of mRNAs of the GA-responsive GASA1 gene, as well as the GA-repressible GA5 gene. Expression studies with cycloheximide indicated that the antagonistic effects of GA and BR on GA5 require de novo protein synthesis. Reporter transgene analyses and RNA-blot analysis showed that BR and GA modulate GA5 expression, at least in part, at the transcriptional level, and that the signals are independent and subtractive.