Bacillus amyloliquefaciens T-5 may prevent Ralstonia solanacearum infection through competitive exclusion
Bacillus amyloliquefaciens T-5 may prevent Ralstonia solanacearum infection through competitive exclusion
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DOI：
10.1007/s00374-015-1079-z
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发表时间：
2016-04-01
影响因子：
6.5
通讯作者：
Xu, Yangchun
中科院分区：
文献类型：
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作者：
Tan, Shiyong;Gu, Yian;Xu, Yangchun
研究根际生防细菌与植物病原菌的相互作用特性和机理，是优化生防策略的必要条件。以番茄为材料，采用不同接种方法，研究了绿色荧光蛋白标记的生防菌解淀粉芽孢杆菌（Bacillus amyloliquefaciens）T-5和红色荧光蛋白标记的青枯病菌（Ralstonia solanacearum）QL-Rs 1115的互作效应。结果表明，在共培养实验中，随着生防菌株初始接种量的增加，病原菌QL-RFP的种群数量逐渐减少。在温室实验中，两种菌株T-5-GFP和QL-RFP定殖番茄根（根尖、根毛、主根和根连接处），并在根表面上形成生物膜，如通过稀释平板和共聚焦激光扫描显微镜（CLSM）技术所确定的。然而，当用T-5-GFP处理土壤和植物幼苗时，在生防菌株T-5-GFP的存在下，病原菌菌株QL-RFP的根定殖几乎被完全抑制。本研究的结果揭示了菌株B的有效性。研究了解淀粉菌T-5作为番茄枯萎病生防菌株的作用及接种方法对生防菌株筛选的意义。
Investigation of the properties and mechanisms of the interactions of root-colonizing biocontrol bacteria and plant pathogens is necessary to optimize the biocontrol strategies. In the present study, the interaction of a biocontrol strain Bacillus amyloliquefaciens T-5 tagged with a green fluorescent protein marker and a bacterial wilt pathogen Ralstonia solanacearum QL-Rs1115 tagged with red fluorescent protein marker was studied on tomato roots using different inoculation methods. The results showed that in the co-culture experiment, the population of pathogen QL-RFP was decreased by increasing the initial inoculum concentration of biocontrol strain. In the greenhouse experiment, both strains T-5-GFP and QL-RFP colonized tomato roots (root tips, root hairs, primary roots, and root junctions) and formed a biofilm on the root surfaces as determined by dilution plating and confocal laser scanning microscopy (CLSM) techniques. However, the root colonization of pathogen strain QL-RFP was almost completely suppressed in the presence of biocontrol strain T-5-GFP when both soil and plant seedlings were treated with T-5-GFP. The results of this study revealed the effectiveness of strain B. amyloliquefaciens T-5 as a biocontrol agent against tomato wilt pathogen and the significance of inoculation method used to inoculate biocontrol strain.