Differential expression of GABAA receptor subunits in the distinct nuclei of the rat amygdala
Differential expression of GABAA receptor subunits in the distinct nuclei of the rat amygdala
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DOI：
10.1016/j.molbrainres.2005.03.013
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发表时间：
2005-07-29
期刊：
影响因子：
--
通讯作者：
Suzuki, H
中科院分区：
文献类型：
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作者：
Fujimura, J;Nagano, M;Suzuki, H
详细了解不同GABA(A)受体亚基的解剖分布对于了解GABA在单个脑区的生理作用以及开发通过单个GABA受体亚型发挥作用的药物至关重要。由于杏仁核是处理情绪信息的关键大脑结构，在每个核团中具有不同的功能，杏仁核中的GABAA受体是治疗情绪障碍的重要靶点。在这项研究中，我们用定量RT-PCR分析了杏仁核、中央核(Ce)和外侧/基底外侧核(LAIBLA)中所有GABA(A)受体亚单位的表达水平。我们发现在CC和LA/BLA中伽马(2)亚单位mRNA的表达最强，在LA/BLA中α(1)、α(2)和α(3)mRNAs的适度表达，在CE中的α(2)和γ(1)mRNAs的表达，在这两个区域都有弱的α(6)、Rho(2)和Rho(3)mRNAs的表达。我们进一步揭示了在CC和LA/BLA中，α(1)、α(3)、α(5)、γ(1)、γ(2)、Delta、epsilon和theta亚单位mRNAs的表达存在显著差异。杏仁核内GABA(A)受体亚单位表达水平的差异表明，对不同药物的敏感性不同，包括苯二氮卓类药物和麻醉剂。(C)2005 Elsevier B.V.保留所有权利。
Detailed knowledge of the anatomical distribution of different GABA(A) receptor subunits is crucial for understanding the physiological actions of GABA in individual brain areas and for developing drugs acting through the individual GABA receptor subtypes. Since the amygdala is a key brain structure in the processing of emotional information with distinct functions in each nucleus, GABAA receptors in the amygdala are an important target of treatment for emotional disorders. In this study, we analyzed by quantitative RT-PCR the expression levels of all GABA(A) receptor subunits in distinct nuclei of the amygdala, the central (Ce) and the lateral/basolateral (LAIBLA) amygdala. We found the strongest expression of the gamma(2) subunit mRNA in both the Cc and LA/BLA, modest expressions of alpha(1), alpha(2) and alpha(3) mRNAs in the LA/ BLA and alpha(2) and gamma(1) mRNAs in the Ce, and weak expressions of alpha(6), rho(2) and rho(3) mRNAs in both regions. We further revealed the significantly different expressions of alpha(1), alpha(3), alpha(5), gamma(1), gamma(2), delta,epsilon and theta subunit mRNAs in the Cc and LA/BLA. Differences in the expression levels of GABA(A) receptor subunits suggest different sensitivity to a variety of drugs including benzodiazepines and anesthetics in amygdala nuclei with distinct functions. (c) 2005 Elsevier B.V. All rights reserved.