Analysis of urinary drugs of abuse by a multianalyte capillary electrophoretic immunoassay
Analysis of urinary drugs of abuse by a multianalyte capillary electrophoretic immunoassay
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DOI：
10.1016/s0021-9673(99)00115-6
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发表时间：
1999-04-09
影响因子：
4.1
通讯作者：
Thormann, W
中科院分区：
文献类型：
--
作者：
Caslavska, J;Allemann, D;Thormann, W
本文描述了一种新的多分析物竞争结合、电动毛细管免疫分析方法，用于尿中美沙酮、鸦片类药物、苯甲酰ecGonine(可卡因代谢物)和苯丙胺的检测。25亩L尿与反应物在有内标的情况下孵育几分钟后，将一小份混合物加入熔融石英毛细管上，用毛细管电泳柱上激光诱导荧光检测来监测未结合的荧光素标记的药物示踪剂。该多分析方法具有快速、简便、定量的特点，能够识别大于或等于30 ng/ml的尿药浓度，适用于患者尿液的筛查。数据显示，与基于酶增殖免疫分析技术和荧光偏振免疫分析的常规筛查方法获得的数据进行了很好的比较。通过对体外质控尿液的分析和患者尿液的GC-MS确证分析，验证了电动毛细管电泳法的有效性。(C)1999 Elsevier Science B.V.保留所有权利。
This paper characterizes a novel multianalyte competitive binding, electrokinetic capillary-based immunoassay for urinary methadone, opiates, benzoylecgonine (cocaine metabolite) and amphetamines. After incubation of 25 mu l urine with the reactants for several minutes in the presence of an internal standard, a small aliquot of the mixture is applied onto a fused-silica capillary and the unbound fluorescein labelled drug tracers are monitored by capillary electrophoresis with on-column laser induced fluorescence detection. The multianalyte assay is shown to be rapid, simple, quantitative, capable of recognizing urinary drug concentrations greater than or equal to 30 ng/ml and suitable for screening of patient urines. Data are demonstrated to compare well with those obtained by routine screening methods based on enzyme multiplied immunoassay techniques and fluorescence polarization immunoassays. The electrokinetic capillary assay has been validated via analysis of external quality control urines and confirmation analysis of patient urines using GC-MS. (C) 1999 Elsevier Science B.V. All rights reserved.