Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand
Natural human humoral response to salivary gland proteins of Anopheles mosquitoes in Thailand
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DOI：
10.1016/j.actatropica.2006.02.004
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发表时间：
2006-04-01
期刊：
影响因子：
2.7
通讯作者：
Udomsangpetch, R
中科院分区：
文献类型：
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作者：
Waitayakul, A;Somsri, S;Udomsangpetch, R
在吸血过程中，节肢动物媒介将唾液注入脊椎动物宿主体内。唾液具有复杂的生物化学和药理活性，可能在病原体传播中发挥重要作用。为了研究蚊子唾液在自然条件下是否能引起人的体液免疫反应，我们收集了疟疾患者、健康村民和泰国一个非疟疾地区的人的血清。在这里，我们已经证明抗唾液按蚊蛋白抗体主要出现在急性恶性疟原虫或间日疟患者中，而来自非疟疾地区的人则没有这种抗体。此外，疟疾患者针对蚊子唾液蛋白的抗体水平变化很大，这可能与蚊子暴露水平有关。尽管存在差异，但高IgG滴度患者的血清一致检测到病毒按蚊唾液腺蛋白提取物中的几种蛋白质。用人血清对按蚊唾液腺进行免疫组化染色，发现唾液腺特异性igg与正中叶反应强烈。按蚊和唾液伊蚊蛋白比较表明，疟疾患者血清中检测到的抗唾液蛋白抗体为按蚊特异性抗体，与疟蚊主要媒介地位一致。这个区域有病毒。(c) 2006 Elsevier B.V.版权所有
During blood feeding, arthropod vectors inject saliva into vertebrate hosts. The saliva is biochemically complex and pharmacologically active, and may play an important role in pathogen transmission. To examine whether mosquito saliva could elicit humoral immune response in humans under natural conditions, we have collected sera from malaria patients, healthy villagers, and people from a non-malarious region in Thailand. Here we have demonstrated that anti-Anopheles salivary protein antibodies occurred predominantly in patients with acute Plasmodium falciparum or P vivax malaria, whereas people from a non-malarious area had no such antibodies. Besides, antibody levels against mosquito salivary proteins in malaria patients were highly variable, which may be related to the levels of mosquito exposure. Despite variability, patients' sera with high IgG titers consistently detected several proteins in Anopheles dirus salivary gland protein extracts. Immunohistochemical staining of Anopheles salivary glands with human sera showed that the salivary gland-specific IgGs reacted strongly with the median lobe. Comparison using Anopheles and Aedes salivary proteins suggests that the anti-salivary protein antibodies detected in malaria patients were Anopheles-specific, consistent with the major malaria vector status of An. dirus in this area. (c) 2006 Elsevier B.V. All rights reserved.