Construction of a micro/nano structured surface on a beta-TCP/CaSiO3 bioceramic promotes osteogenic differentiation of mBMSCs
Construction of a micro/nano structured surface on a beta-TCP/CaSiO3 bioceramic promotes osteogenic differentiation of mBMSCs
复制标题
在β-TCP/CaSiO3生物陶瓷上构建微/纳米结构表面促进mBMSCs的成骨分化
DOI：
10.1039/c8ce01711e
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发表时间：
2019
期刊：
影响因子：
3.1
通讯作者：
Wang Yingjun
中科院分区：
文献类型：
--
作者：
Liu Xiao;Zhao Naru;Guo Xiaoheng;Duan Haibo;Diao Jingjing;Dong Yifan;Wang Yingjun
细胞行为是对生物材料，特别是材料表面的同源反应的结果。一种有效的方法来引起所需的生物反应是采用微/纳米结构的生物材料表面设计的结构，然而，无机材料的脆性通常阻碍结构调节。在这项研究中，微/纳米结构的表面制造使用水热处理，不需要晶体调节试剂。将前体β-磷酸三钙与CaSiO 3混合。所得的微/纳米结构表面具有包含纳米线、微米棒和棒簇的混合结构。体外细胞增殖、细胞毒性和成骨分化实验表明，微/纳米结构表面减轻了材料的细胞毒性，提高了细胞增殖能力，有效促进了小鼠骨髓间充质干细胞的成骨分化。这些结果表明，这种简化的表面处理是提高细胞对无机生物材料的生物学反应的有效途径。
Cellular behavior is a result of homologous responses to biomaterials, especially material surfaces. An effective way to elicit a desired biological response is to employ micro/nanostructured constructions for biomaterial surface design; however, the brittleness of inorganic materials usually hinders structure regulation. In this study, a micro/nanostructured surface was fabricated using a hydrothermal treatment that did not require crystal regulation reagents. The precursor, β-tricalcium phosphate, was mixed with CaSiO3. The resulting micro/nanostructured surface had a hybrid structure containing nanowires, microrods, and rod clusters. In vitro cellular proliferation, cytotoxicity and osteodifferentiation experiments indicated that the micro/nanostructured surfaces alleviated material cytotoxicity, improved cell proliferation, and effectively promoted osteogenic differentiation of mouse bone mesenchymal stem cells. These results suggest that this simplified surface treatment is an efficient way to improve cell biological response to inorganic biomaterials.