Sequence-selective, pH-dependent binding to DNA of benzophenanthridine alkaloids.
Sequence-selective, pH-dependent binding to DNA of benzophenanthridine alkaloids.
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DOI：
10.1002/jmr.300030106
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发表时间：
1990-02-01
期刊：
影响因子：
--
通讯作者：
Smekal, E
中科院分区：
文献类型：
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作者：
Bajaj, N P;McLean, M J;Smekal, E
用DNase I足迹法分析了苯并菲并吡啶类三种生物碱与DNA结合时的序列选择性，并与标准插层剂溴化乙锭的行为分析结果进行了比较。与乙锭一样，苯并菲并菲类化合物似乎与混合核苷酸序列的区域结合得最好，特别是那些含有交替的嘌呤和嘧啶的区域，尽管在行为上有一些显著的差异。也明显缺乏与序列如(AT)n的结合，其中n大于或等于3。苯并菲并菲与DNA的结合与介质的氢离子浓度特别相关，因为当在pH低于7.0的条件下进行反应时，DNase I的足迹显著增强。我们根据插层物种在较低pH时存在的更大优势来讨论这些结果。
The sequence selectivity associated with binding to DNA of three alkaloids belonging to the benzophenanthridine family has been analysed by DNase I footprinting, and the results were compared with those obtained from an analysis of the behaviour of the standard intercalator, ethidium bromide. Like the ethidium, the benzophenanthridine compounds appear to bind best to regions of mixed nucleotide sequence, especially those containing alternating purines and pyrimidines, although there are some notable differences in behaviour. There is also a marked lack of binding to sequences such as (AT)n, where n greater than or equal to 3. The binding to DNA of the benzophenanthridines is specifically related to the hydrogen ion concentration of the medium, in that the DNase I footprints are considerably enhanced when the reaction is performed at a pH below 7.0. We discuss these results in terms of a greater preponderance of the intercalating species being present at lower pH.