Elucidation of the Pathogenicity-Associated Regulatory Network in Xanthomonas oryzae pv. oryzae.
Elucidation of the Pathogenicity-Associated Regulatory Network in Xanthomonas oryzae pv. oryzae.
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DOI：
10.1128/msystems.00789-20
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发表时间：
2021-03-09
期刊：
影响因子：
6.4
通讯作者：
Ruan L
中科院分区：
文献类型：
--
作者：
Zheng D;Wang H;Zhong H;Ke W;Hu H;Sun M;Ruan L
黄单胞菌属是一种臭名昭著的植物病原菌，可引起数百种植物寄主的严重病害。黄单胞菌属物种配备有一系列信号转导系统，调节基因表达以在各种恶劣环境中生存并成功感染宿主。虽然某些致病性相关的调节器已被功能上的特点，信号转导系统总是作为一个调控网络，仍有待阐明在黄单胞菌。本研究采用了系统的方法来表征所有确定的致病性相关的监管机构在黄单胞菌pv。Xoo基因及其相互作用的调控网络。RNA测序用于阐明所确定的10种病原性相关调节因子的模式。结果表明，每个致病性相关调节因子之间都存在相互干扰，所有这些调节因子组成一个调控网络，其中VemR和PXO_RS20790是致病性相关的主要调节因子，HrpX是最终的执行因子。此外，调节组分析表明，许多基因以外的致病性岛的基因的调控网络内进行精细的调节。鉴于大多数致病性相关的监管机构是保守的黄单胞菌目，我们的研究结果表明，在这个进化保守的病原体的基因调控的全球网络。总之，我们的研究提供了关于Xoo中调控网络的基本信息，表明这种复杂的调控网络是黄单胞菌稳健性和适应性的原因之一。病原菌侵染寄主植物的过程是一个协调的细胞行为，需要在多个水平上进行动态调节，以响应寄主植物的变化或外部环境的波动。黄单胞菌属是植物病原细菌中最重要的一个属，一直被作为模式菌进行研究。虽然某些致病性相关的监管机构已功能特点，它们之间的相互作用仍有待阐明。本研究系统地表征了Xoo中的致病性相关调节子，并揭示了致病性相关调节子之间存在串扰，并且作为调节网络发挥作用，其中调节子之间存在层次结构。我们的研究阐明了黄单胞菌致病性相关的调控网络的景观，促进了解致病菌的感染过程。
Xanthomonas is a notorious plant pathogen causing serious diseases in hundreds of plant hosts. Xanthomonas species are equipped with an array of signal transduction systems that regulate gene expression to survive in various harsh environments and successfully infect hosts. Although certain pathogenicity-associated regulators have been functionally characterized, signal transduction systems always function as a regulatory network which remains to be elucidated in Xanthomonas. This study used a systematic approach to characterize all identified pathogenicity-associated regulators in Xanthomonas oryzae pv. oryzae (Xoo), including a transcriptional regulator with unknown function, and their interactive regulatory network. RNA sequencing was used in elucidating the patterns of the 10 pathogenicity-associated regulators identified. Results revealed that each pathogenicity-associated regulator has cross talk with others and all these regulators function as a regulatory network, with VemR and PXO_RS20790 being the master pathogenicity-associated regulators and HrpX being the final executant. Moreover, regulome analysis showed that numerous genes other than genes in pathogenicity islands are finely regulated within the regulatory network. Given that most of the pathogenicity-associated regulators are conserved in Xanthomonadales, our findings suggest a global network of gene regulation in this evolutionarily conserved pathogen. In conclusion, our study provides essential basic information about the regulatory network in Xoo, suggesting that this complicated regulatory network is one of the reasons for the robustness and fitness of Xanthomonas spp. IMPORTANCE The host plant infection process of pathogenic bacteria is a coordinating cellular behavior, which requires dynamic regulation at several levels in response to variations in host plants or fluctuations in the external environment. As one of the most important genera of plant-pathogenic bacteria, Xanthomonas has been studied as a model. Although certain pathogenicity-associated regulators have been functionally characterized, interactions among them remain to be elucidated. This study systematically characterized pathogenicity-associated regulators in Xoo and revealed that cross talk exists among pathogenicity-associated regulators and function as a regulatory network in which a hierarchy exists among the regulators. Our study elucidated the landscape of the pathogenicity-associated regulatory network in Xanthomonas, promoting understanding of the infection process of pathogenic bacteria.