Participation of Chromatin-Remodeling Proteins in the Repair of Ultraviolet-B-Damaged DNA
Participation of Chromatin-Remodeling Proteins in the Repair of Ultraviolet-B-Damaged DNA
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DOI：
10.1104/pp.111.191452
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发表时间：
2012-02-01
期刊：
影响因子：
7.4
通讯作者：
Casati, Paula
中科院分区：
文献类型：
--
作者：
Campi, Mabel;D'Andrea, Lucio;Casati, Paula
植物的基因组被组织成染色质，影响转录、DNA重组和修复的速率。在这项工作中，我们已经调查了一些染色质重塑因子的表达减少和组蛋白乙酰化在玉米（玉米）和拟南芥（拟南芥）在他们参与DNA修复后，紫外线（UV）-B照射的后果。缺乏NFC102/NFC4或SDG102/SDG26的植物显示出比野生型植物更多的DNA损伤;然而，拟南芥chc1突变体显示出与野生型植物相似的环丁烷嘧啶二聚体积累，与玉米chc101 RNA干扰系中测得的DNA损伤增加相反。在拟南芥中，缺乏染色质重塑的植物也会受到UV-B的影响而积累色素。在UV-B处理之前用组蛋白乙酰转移酶抑制剂姜黄素处理的植物显示出DNA修复的缺陷;此外，染色质重塑缺陷植物在UV-B处理后改变了乙酰化组蛋白的水平，表明组蛋白乙酰化在这两种植物物种的DNA修复过程中是重要的。拟南芥突变体ham1和ham2也表现出增加UV-B后的DNA损伤，这表明这些蛋白在DNA损伤修复中的作用已经通过进化而保守。然而，环丁烷嘧啶二聚体的积累是较高的ham1比ham2，这表明HAM1在UV-B后的DNA修复中发挥了重要作用。总之，在这项工作中，我们已经证明，染色质重塑，特别是组蛋白乙酰化，是重要的DNA修复过程中的UV-B，表明遗传和表观遗传效应控制DNA修复植物。
The genome of plants is organized into chromatin, affecting the rates of transcription, DNA recombination, and repair. In this work, we have investigated the consequences of reduced expression of some chromatin-remodeling factors and histone acetylation in maize (Zea mays) and Arabidopsis (Arabidopsis thaliana) in their participation in DNA repair after ultraviolet (UV)-B irradiation. Plants deficient in NFC102/NFC4 or SDG102/SDG26 showed more damaged DNA than wild-type plants; however, the Arabidopsis chc1 mutant showed similar accumulation of cyclobutane pyrimidine dimers as wild-type plants, in contrast to the increased DNA damage measured in the maize chc101 RNA interference line. In Arabidopsis, plants deficient in chromatin remodeling are also affected in the accumulation of pigments by UV-B. Plants treated with an inhibitor of histone acetyltransferases, curcumin, previous to the UV-B treatment show deficiencies in DNA repair; in addition, the chromatin remodeling-deficient plants have altered levels of acetylated histones after the UV-B treatment, demonstrating that histone acetylation is important during DNA repair in these two plant species. Arabidopsis mutants ham1 and ham2 also showed increased DNA damage after UV-B, suggesting that the role of these proteins in DNA damage repair has been conserved through evolution. However, cyclobutane pyrimidine dimer accumulation was higher in ham1 than in ham2; suggesting that HAM1 has a major role in DNA repair after UV-B. In summary, in this work, we have demonstrated that chromatin remodeling, and histone acetylation in particular, is important during DNA repair by UV-B, demonstrating that both genetic and epigenetic effects control DNA repair in plants.