Regional effect of naltrexone in the nucleus of the solitary tract in blockade of NPY-induced feeding
Regional effect of naltrexone in the nucleus of the solitary tract in blockade of NPY-induced feeding
复制标题
DOI：
10.1152/ajpregu.2000.278.2.r499
复制
发表时间：
2000-02-01
影响因子：
2.8
通讯作者：
Billington, CJ
中科院分区：
文献类型：
--
作者：
Kotz, CM;Glass, MJ;Billington, CJ
孤束核(NTS)内的纳曲酮(NLTX)可减弱室旁核(PVN)神经肽Y(NPY)的摄食作用。我们试图确定对NLTX阻断PVN最敏感的NTS区域：NPY诱导的摄食。雄性SD大鼠在下丘脑室旁核(PVN)和后脑区(NTS的尾侧、内侧或吻部，或NTS外1 mm)安装了两个插管。在PVN NPY(0.5mg0.3亩L)或人工脑脊液(0.3mL)之前，动物后脑区注射NLTX(0、1、3、10和30 mg的0.3亩L)。在注射后2小时测量摄食量。PVN NPY刺激摄食，内侧NTS的NLTX在2 h显著降低NPY诱导的摄食，其他后脑区给予NLTX对PVN NPY诱导的摄食无明显影响。这些数据表明，刺激NPY后，内侧NTS中的阿片受体对起源于PVN的馈入信号反应最快。
Naltrexone (NLTX) in the nucleus of the solitary tract (NTS) decreases feeding induced by neuropeptide Y (NPY)in the paraventricular nucleus (PVN). We sought to determine the NTS region most sensitive to NLTX blockade of PVN:NPY-induced feeding. Male Sprague-Dawley rats were fitted with two cannulas; one in the PVN and one in a hindbrain region: caudal, medial, or rostral NTS or 1 mm outside the NTS. Animals received NLTX (0, 1, 3, 10, and 30 mu g in 0.3 mu l) into the hindbrain region just prior to PVN NPY (0.5 mu g, 0.3 mu l) or artificial cerebrospinal fluid (0.3 mu l). Food intake was measured at 2 h following injection. PVN NPY stimulated feeding, and NLTX in the medial NTS significantly decreased NPY-induced feeding at 2 h, whereas administration of NLTX in the other hindbrain regions did not significantly influence PVN NPY induced feeding. These data suggest that opioid receptors in the medial NTS are most responsive to feeding signals originating in the PVN after NPY stimulation.