Hyperosmolaric contrast agents in cartilage tomography may expose cartilage to overload-induced cell death
Hyperosmolaric contrast agents in cartilage tomography may expose cartilage to overload-induced cell death
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DOI：
10.1016/j.jbiomech.2011.11.049
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发表时间：
2012-02-02
影响因子：
2.4
通讯作者：
Korhonen, R. K.
中科院分区：
文献类型：
--
作者：
Turunen, M. J.;Toyras, J.;Korhonen, R. K.
在临床关节造影检查中，强高渗造影剂直接注入关节间隙。这可能会降低关节软骨的僵硬，进一步假设这会导致超负荷诱导的细胞死亡。我们观察了在生理盐水溶液和全强度高渗X射线造影剂Hexabrix(TM)中原位压迫关节软骨时软骨细胞的死亡情况。标本取自牛的膝盖骨，在Dulbecco‘s改良的Eagle’s培养液中保存过夜。在此基础上，对接触应力和蠕变时间分别为1~10和0~15min的试件进行了有蠕变和无蠕变的冲击试验。最后，用共聚焦显微镜检测细胞的存活率。为了研究Hexabrix(TM)对软骨生物力学性能的影响，对浸泡在Hexabrix(TM)和磷酸盐缓冲盐水(PBS)中的试件进行了应力松弛试验。浸泡在Hexabrix(TM)中的样品的动态模和平衡模量均显著低于浸泡在PBS中的样品(p<；0.05)。浸泡在生理盐水中的软骨样本显示，负载诱导的细胞死亡主要发生在浅层和中层。然而，在8-10 Mpa的高接触应力下，全强度Hexabrix(TM)浸泡的样本显示的死亡细胞数显著高于在生理盐水中压缩的样本(p<；0.05)，尤其是在软骨深层。结论：在高渗造影剂作用下，过大的载荷应力和组织蠕变可能会增加关节软骨细胞死亡的风险，尤其是在软骨深层。(C)2011爱思唯尔有限公司。保留所有权利。
In clinical arthrographic examination, strong hypertonic contrast agents are injected directly into the joint space. This may reduce the stiffness of articular cartilage, which is further hypothesized to lead to overload-induced cell death. We investigated the cell death in articular cartilage while the tissue was compressed in situ in physiological saline solution and in full strength hypertonic X-ray contrast agent Hexabrix (TM). Samples were prepared from bovine patellae and stored in Dulbecco's Modified Eagle's Medium overnight. Further, impact tests with or without creep were conducted for the samples with contact stresses and creep times changing from 1 MPa to 10 MPa and from 0 min to 15 min, respectively. Finally, depth-dependent cell viability was assessed with a confocal microscope. In order to characterize changes in the biomechanical properties of cartilage as a result of the use of Hexabrix (TM), stress-relaxation tests were conducted for the samples immersed in Hexabrix (TM) and phosphate buffered saline (PBS). Both dynamic and equilibrium modulus of the samples immersed in Hexabrix (TM) were significantly (p < 0.05) lower than those of the samples immersed in PBS. Cartilage samples immersed in physiological saline solution showed load-induced cell death primarily in the superficial and middle zones. However, under high 8-10 MPa contact stresses, the samples immersed in full strength Hexabrix (TM) showed significantly (p < 0.05) higher number of dead cells than the samples compressed in physiological saline, especially in the deep zone of cartilage. In conclusion, excessive loading stresses followed by tissue creep might increase the risk for chondrocyte death in articular cartilage when immersed in hypertonic X-ray contrast agent, especially in the deep zone of cartilage. (C) 2011 Elsevier Ltd. All rights reserved.