Meeting report: Batch-to-batch variability in estrogenic activity in commercial animal diets - Importance and approaches for laboratory animal research
Meeting report: Batch-to-batch variability in estrogenic activity in commercial animal diets - Importance and approaches for laboratory animal research
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DOI：
10.1289/ehp.10524
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发表时间：
2008-03-01
影响因子：
10.4
通讯作者：
Saal, Frederick S. vom
中科院分区：
文献类型：
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作者：
Heindel, Jerrold J.;Saal, Frederick S. vom
我们报告了来自美国国立卫生研究院主办的两个研讨会的信息，这两个研讨会的目的是：a）评估膳食雌激素是否会显著影响实验动物的终点; B）让项目参与者和饲料制造商参与进来，解决与测量和消除啮齿动物饮食批次间差异相关的问题，这些差异可能导致实验室内和实验室间动物实验中出现相互矛盾的结果。研讨会上介绍的数据表明，商业动物饲料中雌激素含量存在显著的批次间差异，这种差异导致实验结果的差异。提出了一种方法的组合，以确定总雌激素活性的水平和特定的雌激素成分的水平，含大豆，酪蛋白，和其他大豆无啮齿动物的饮食。研讨会与会者建议研究人员更加注意动物研究中使用的饮食类型，并选择其雌激素活性（或缺乏雌激素活性）适合实验模型和终点的饮食。关于特定植物雌激素水平的信息，以及由其他污染物引起并通过生物测定法测量的雌激素活性，应在科学出版物中披露。这将要求实验室动物饲料制造商向研究人员提供有关动物研究中使用的商业饲料中植物雌激素含量和其他雌激素化合物的信息。
We report information from two workshops sponsored by the National Institutes of Health that were held to a) assess whether dietary estrogens could significantly impact end points in experimental animals, and b) involve program participants and feed manufacturers to address the problems associated with measuring and eliminating batch-to-batch variability in rodent diets that may lead to conflicting findings in animal experiments within and between laboratories. Data were presented at the workshops showing that there is significant batch-to-batch variability in estrogenic content of commercial animal diets, and that this variability results in differences in experimental outcomes. A combination of methods were proposed to determine levels of total estrogenic activity and levels of specific estrogenic constituents in soy-containing, casein-containing, and other soy-free rodent diets. Workshop participants recommended that researchers pay greater attention to the type of diet being used in animal studies and choose a diet whose estrogenic activity (or lack thereof) is appropriate for the experimental model and end points of interest. Information about levels of specific phytoestrogens, as well as estrogenic activity caused by other contaminants and measured by bioassay, should be disclosed in scientific publications. This will require laboratory animal diet manufacturers to provide investigators with information regarding the phytoestrogen content and other estrogenic compounds in commercial diets used in animal research.