Expression of Human Interleukin 8 in Mice Alters Their Natural Behaviors.
Expression of Human Interleukin 8 in Mice Alters Their Natural Behaviors.
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DOI：
10.2147/jir.s355669
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发表时间：
2022
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
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作者：
研究人白细胞介素（IL）8表达对小鼠行为的影响。
构建了一种在椎间盘（IVD）和软骨组织中表达人IL8的小鼠品系（hIL8 +）。利用全自动、非侵入性平台记录小鼠的自发行为，包括移动、攀爬、直立、梳理、进食、饮水和静止不动。
与同窝对照小鼠相比，hIL8 +小鼠移动的距离随年龄增长而减少，并且雄性hIL8 +小鼠移动的距离比雄性对照小鼠以及两种基因型的雌性小鼠都短（p <0.05）。hIL8 +小鼠用于移动的时间也比对照小鼠少（p <0.01），并且在12周龄及以上时，雄性hIL8 +小鼠用于移动的时间最少，移动次数也最低，与其他3组相比（p <0.05）。hIL8 +小鼠用于攀爬的时间比对照小鼠少，并且雄性小鼠用于攀爬的时间比相同基因型的雌性小鼠少（p <0.01）。hIL8 +小鼠用于进食的时间比对照小鼠多，用于饮水的时间比对照小鼠少，并且所有小鼠随着年龄增长用于进食的时间减少，用于饮水的时间增加。最后，hIL8 +小鼠静止不动的时间比对照小鼠多，并且雄性hIL8 +小鼠静止不动的时间比其他任何一组都多（p <0.05）。
hIL8 +小鼠，尤其是雄性hIL8 +小鼠，表现出移动和攀爬减少。小鼠表现出与年龄相关的进食减少以及饮水和梳理时间增加，这也受到hIL8表达的影响。对照小鼠自然行为的这些变化与年龄增长导致的功能衰退是一致的。hIL8对自然衰老过程的影响可能涉及全身性（例如，对大脑）和局部性（例如，在脊柱和关节组织中）机制。对这些机制的进一步探索可能会有成效。
To examine the effects of human interleukin (IL) 8 expression on mouse behavior. A mouse line expressing human IL8 in the intervertebral discs (IVD) and cartilaginous tissues (hIL8+) was generated. Mouse spontaneous behaviors, including locomotion, climbing, rearing, grooming, eating, drinking, and immobility were recorded with a fully automatic, non-invasive platform. Distance traveled by the hIL8+ mice declined with age compared with control littermates, and male hIL8+ mice traveled a shorter distance than male controls and females of either genotype (p <0.05). The hIL8+ mice also spent less time in locomotion than control mice (p <0.01), and male hIL8+ mice spent the least amount of time and had lowest count in locomotion compared with the other 3 groups at 12 weeks of age or greater (p <0.05). The hIL8+ mice spent less time climbing than controls, and male mice spent less time climbing than female mice of the same genotype (p <0.01). The hIL8+ mice spent more time eating and less time drinking than controls, and all mice spent less time eating and more time drinking with increasing age. Finally, hIL8+ mice spent more time immobile than controls, and male hIL8+ mice spent more time immobile than any other group (p <0.05). The hIL8+ mice, especially hIL8+ males, showed reduced ambulation and climbing. Mice showed age-related decrease in eating and increase in drinking and grooming time that was also influenced by expression of hIL8. These changes in natural behaviors in control mice are consistent with functional decline with age. Effects of hIL8 superimposed on the natural aging process could involve systemic (e.g., on the brain) and local (e.g., in the spine and joint tissues) mechanisms. Future exploration of these mechanisms might be productive.