Genomic organization and tissue expression of the murine gene encoding the protein beta-aspartate methyltransferase.
Genomic organization and tissue expression of the murine gene encoding the protein beta-aspartate methyltransferase.
复制标题
编码蛋白质β-天冬氨酸甲基转移酶的小鼠基因的基因组组织和组织表达。
DOI：
10.1016/0378-1119(92)90191-q
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
O'Connor,CM
中科院分区：
文献类型：
--
作者：
Romanik,EA;Ladino,CA;Killoy,LC;D'Ardenne,SC;O'Connor,CM
从基因组文库中分离到两个重叠的克隆，其中包含编码小鼠蛋白β-天冬氨酸甲基转移酶（EC 2.1.1.77）的整个684核苷酸序列。对两个克隆的部分序列分析表明，PCMT编码序列分布在基因组DNA 25 kb内的7个外显子中，长度在32 ~ 339 bp之间。三个外显子对应的一级结构区域在许多真核和原核酶中是高度保守的，这些酶利用-腺苷蛋氨酸（AdoMet）和-腺苷同型半胱氨酸（AdoHcy）。PCMT编码基因（PCMT）的5'侧区包含一个800 bp的富含G+ c的区域，具有转录因子ETF的潜在结合位点，但缺乏TATA盒和其他已知转录因子的结合位点。在小鼠脑、睾丸、肝脏和肾脏提取的RNA的Northern印迹上检测到多个epcmtmrna。每个组织中pcmtmrna的总体丰度与PCMT的测量特异性活性平行。将pcmtmrna的基因组序列信息与小鼠睾丸文库中两个cDNA克隆的3′-非翻译区（UTRs）进行比较，表明pcmtmrna前体经历了选择性剪接。pcmt1的结构和广泛表达是脊椎动物看家基因的特征。
Two overlapping clones containing the entire 684-nucleotide (nt) sequence encoding murine protein β-aspartate methyltransferase (EC 2.1.1.77) were isolated from a genomic library. Partial nt sequence analysis of the two clones revealed that the protein carboxyl methyltransferase (PCMT)-encoding sequence is distributed among seven exons, ranging from 32 to 339 bp in length, within 25 kb of genomic DNA. Three exons correspond to regions of primary structure which are strongly conserved among a number of eukaryotic and prokaryotic enzymes which utilizeS-adenosylmethionine (AdoMet) andS-adenosylhomocysteine (AdoHcy). The 5'-flanking region of the PCMT-encoding gene (PCMT) contains an 800-bp G+C-rich region with potential binding sites for transcription factor ETF, but lacks a TATA box and binding sites for other known transcription factors. MultiplePCMTmRNAs were detected on Northern blots of RNA extracted from murine brain, testis, liver and kidney. The overall abundance ofPCMTmRNAs in each tissue paralleled the measured specific activity of the PCMT. Comparison of the genomic sequence information with the 3'-untranslated regions (UTRs) of two cDNA clones from a murine testis library indicated thatPCMTmRNA precursors undergo alternative splicing. The structure and widespread expression ofPCMTare characteristics of vertebrate housekeeping genes.