Analysis of hypocretin (orexin) antibodies in patients with narcolepsy
Analysis of hypocretin (orexin) antibodies in patients with narcolepsy
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DOI：
10.1093/sleep/28.4.427
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发表时间：
2005-04-01
期刊：
影响因子：
5.6
通讯作者：
Slocumb, NL
中科院分区：
文献类型：
--
作者：
Black, JL;Silber, MH;Slocumb, NL
研究目的：我们验证了发作性睡病患者血清中有抗前原分泌素及其衍生物特异性抗体的假设。设计：采用酶联免疫吸附试验(EL ISA)检测HLADQB1*0602阳性发作性睡病患者血清中抗人前降钙素原、1和2、N端前导和C端多肽的自身抗体的证据。这些结果与非发作性精神障碍和睡眠呼吸暂停对照组的样本进行了比较。实验室人员对受试者的状态和环境视而不见：从明尼苏达州罗切斯特、亚利桑那州斯科茨代尔和佛罗里达州杰克逊维尔的梅奥诊所招募发作性睡病患者和非发作性发作性睡病患者。实验室测试在罗切斯特梅奥诊所的梅奥心理基因组实验室进行。参与者：34名发作性睡病患者和49名非发作性发作性睡病患者的样本。干预：无。测量和结果：ELISA测量光密度。初步分析了发作性睡病患者和对照组的每个潜在抗原，在Bonferroni调整后，没有一个差异达到显著性所需的P值。按年龄和性别进行二次分析，只有2例患者在Bonferroni校正后的P值显著，但进一步的统计分析对这些差异的准确性提出了怀疑。在所有记录有显著差异的病例中，由于对照光密度读数高于发作性睡眠值，该假说不被支持。结论：这些ELISA法结果不支持HLADQB1*0602阳性发作性睡病与血清中抗前降钙素或其裂解产物抗体有关的假说。
Study Objectives: We tested the hypothesis that patients with narcolepsy have serum antibodies specific for preprohypocretin and its derivatives. Design: We tested sera from strictly diagnosed HLA DQB1*0602-positive narcoleptic patients with cataplexy for evidence of autoantibodies against human preprohypocretin, hypocretin 1 and 2, N-terminal leader and C-terminal peptides of preprohypocretin using enzyme-linked immunosorbent assays (ELISA). These results were compared to samples from nonnarcoleptic psychiatric and sleep apnea controls. Laboratory personnel were blinded to subject status,Setting: Narcoleptic patients and nonnarcoleptic controls were recruited from the Mayo Clinic facilities in Rochester, Minnesota; Scottsdale, Arizona; and Jacksonville, Florida. Laboratory testing was conducted in the Mayo Psychogenomic Laboratory at the Rochester Mayo Clinic.Participants: A sample of 34 narcoleptic patients and 49 nonnarcoleptic controls.Interventions: None.Measurements and Results: ELISA measurements were in optical density. Primary analyses were of the entire narcoleptic and control groups for each potential antigen, and none of the differences reached P values required for significance after Bonferroni adjustment. Secondary analyses by age and sex yielded P values that were significant after Bonferroni adjustment in only 2 cases, but further statistical analyses cast doubt on the veracity of these differences. In all cases where a significant difference was recorded, the hypothesis was not supported because the control optical density reading was higher than the narcoleptic values.Conclusions: These ELISA assay results do not support the hypothesis that HLA DQB1*0602-positive narcolepsy with cataplexy is associated with serum antibodies against preprohypocretin or its cleavage products.