Mutations affecting the meiotic and mitotic divisions of the early Caenorhabditis elegans embryo.
Mutations affecting the meiotic and mitotic divisions of the early Caenorhabditis elegans embryo.
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影响早期秀丽隐杆线虫胚胎减数分裂和有丝分裂的突变。
DOI：
10.1093/genetics/126.3.593
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Wood,WB
中科院分区：
文献类型：
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作者：
Mains,PE;Kemphues,KJ;Sprunger,SA;Sulston,IA;Wood,WB
我们描述了秀丽隐杆线虫的四个基因中的母效应致死突变之间的相互作用，其产物似乎参与了单细胞胚胎的减数分裂和有丝分裂。有丝分裂被两个显性的温度敏感性功能获得性母源效应致死突变mei-1（ct 46）和mel-26（ct61）以及zyg-9的隐性功能丧失性母源效应致死突变破坏。由这些突变引起的表型缺陷是相似的。双突变体的组合显示出强烈的增强的母效应的致死率在半容许条件下，这表明突变基因产物相互作用。我们分离了15个功能获得性突变mei-1（ct 46）的显性抑制基因。这些抑制因子中有13个是基因内的，但其中11个抑制反式和顺式。两个基因外抑制子定义了一个新基因mei-2。这两个基因的抑制突变也导致隐性母性效应致死，但伴随减数分裂缺陷而不是有丝分裂缺陷。令人惊讶的是，除了mei-1（ct 46）之外，这些抑制剂中的大多数还能够抑制mel-26（ct61）。四个基因mei-1，mei-2，zyg-9和mel-26的产物可能负责区分单细胞胚胎中减数分裂和有丝分裂的一些特化特征。
We describe interactions between maternal-effect lethal mutations in four genes of Caenorhabditis elegans whose products appear to be involved in the meiotic and mitotic divisions of the one-cell embryo. Mitosis is disrupted by two dominant temperature-sensitive gain-of-function maternal-effect lethal mutations, mei-1(ct46) and mel-26(ct61), and by recessive loss-of-function maternal-effect lethal mutations of zyg-9. The phenotypic defects resulting from these mutations are similar. Doubly mutant combinations show a strong enhancement of the maternal-effect lethality under semipermissive conditions, suggesting that the mutant gene products interact. We isolated 15 dominant suppressors of the gain-of-function mutation mei-1(ct46). Thirteen of these suppressors are apparently intragenic, but 11 of them suppress in trans as well as cis. Two extragenic suppressors define a new gene, mei-2. The suppressor mutations in these two genes also result in recessive maternal-effect lethality, but with meiotic rather than mitotic defects. Surprisingly, most of these suppressors are also able to suppress mel-26(ct61) in addition to mei-1(ct46). The products of the four genes mei-1, mei-2, zyg-9 and mel-26 could be responsible for some of the specialized features that distinguish the meiotic from the mitotic divisions in the one-cell embryo.
细胞外基质的分子生物物理学
DOI：
--
发表时间：
1985
期刊：
影响因子：
--
作者：
A. Perlin
通讯作者：
A. Perlin
糖蛋白和蛋白聚糖的生物化学
影响因子：
--
作者：
W. Lennarz
通讯作者：
W. Lennarz