Chitosan monomer accelerates alkaline phosphatase activity on human osteoblastic cells under hypofunctional conditions
Chitosan monomer accelerates alkaline phosphatase activity on human osteoblastic cells under hypofunctional conditions
复制标题
DOI：
10.1002/jbm.a.31234
复制
发表时间：
2007-11-01
影响因子：
4.9
通讯作者：
Hayashi, Yoshihiko
中科院分区：
文献类型：
--
作者：
Yamada, Shizuka;Ganno, Tomoko;Hayashi, Yoshihiko
壳聚糖是一种天然的聚氨基糖，广泛用作抗肿瘤和抗风湿药物。然而，关于其对体外功能低下的成骨细胞的影响的报道很少。我们研究了在模拟微重力条件下用含壳聚糖单体的培养基培养的人成骨细胞系（NOS-1 细胞）的生物学特性。细胞在常规条件下孵育7天后，将烧瓶转移至微重力模拟器中3天。在0.005%壳聚糖单体补充组中，生物矿化的标志酶碱性磷酸酶（ALP）活性显着高于对照组（p < 0.05）。采用cDNA微阵列研究壳聚糖单体对mRNA水平的影响，并分析荧光信号。检测到干扰素γ(IFN-γ)受体基因，信号比为2.2。根据RT-PCR分析，孵育3天后证实IFN-γ受体表达略有增加。蛋白质印迹分析还显示IFN-γ受体的表达增加。这些结果表明，超低浓度的壳聚糖单体可能在细胞培养早期通过IFN-γ受体增加成骨细胞的ALP活性，恢复低功能状态下的生物矿化活性。 (c) 2007 年 Wiley 期刊公司。
Chitosan is a natural polyaminosaccharide that is extensively applied as an antitumor and antirheumatic drug. However, there are few reports about its effects on hypofunctional osteoblasts in vitro. We investigated the biological characteristics of a human osteoblastic cell line (NOS-1 cells) that was cultured with a chitosan monomer-containing medium under simulated microgravity conditions. After 7 days of cell incubation under the conventional conditions, the flasks were transferred to a microgravity simulator for 3 days. In the 0.005% chitosan monomer supplemented group, the marker enzyme of biological mineralization, the alkaline phosphatase (ALP) activity, was significantly higher compared with the control group (p < 0.05). A cDNA microarray was performed to investigate the effects on the mRNA level by chitosan monomer, and the fluorescent signal was analyzed. The interferon gamma (IFN-gamma) receptor gene was detected with a signal ration of 2.2. The slight increase of IFN-gamma receptor expression was confirmed after 3 days of incubation according to RT-PCR analysis. Western blot analysis also showed the increased expression of IFN-gamma receptor. These results suggest that a supra-low concentration of chitosan monomer may increase the ALP activity of osteoblastic cells through the IFN-gamma receptor at the early phase of cell culture and recover the activity for biological mineralization under the hypofunctional condition. (c) 2007 Wiley Periodicals, Inc.