Cytokine control of interstitial collagenase and collagenase-3 gene expression in human chondrocytes
Cytokine control of interstitial collagenase and collagenase-3 gene expression in human chondrocytes
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DOI：
10.1074/jbc.271.38.23577
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发表时间：
1996-09-20
影响因子：
4.8
通讯作者：
Heller, RA
中科院分区：
文献类型：
--
作者：
Borden, P;Solymar, D;Heller, RA
人类胶原酶-3的表达，以前只在乳腺肿瘤组织中看到，这里显示在来自关节组织的原代人类软骨细胞和转化的人类软骨细胞中表达。炎性细胞因子IL-1β和肿瘤坏死因子-α可诱导其基因表达，佛波酯-12-肉豆蔻酸酯-13-乙酸酯不能诱导其表达，生长因子血小板衍生生长因子和表皮生长因子对其有轻微的诱导作用。人类滑膜成纤维细胞是关节组织中另一种重要的细胞类型，它不产生胶原酶-3信息，小鼠胶原酶的表达可能是人胶原酶-3的对应物，由白细胞介素1β和肿瘤坏死因子-α诱导，其完全诱导需要转录因子c-fos的存在，这个转录因子家族也在诱导人胶原酶-3的作用中发挥作用，因为它与这种基质金属蛋白酶的AP-1位点结合。
Human collagenase-3 expression, previously seen only in a breast tumor tissue, is here shown to be expressed in primary human chondrocytes derived from the joint tissue and in transformed human chondrocytes. Its mRNA is inducible by the inflammatory cytokines interleukin-1 beta plus tumor necrosis factor-alpha, but not by phorbol 12-myristate 13-acetate and only slightly by the growth factors platelet-derived growth factor and epidermal growth factor. Human synovial fibroblasts, another prominent cell type in the joint tissue, do not produce collagenase-3 message, Expression of the murine collagenase, which is possibly the counterpart of human collagenase-3, is induced by interleukin-1 beta plus tumor necrosis factor-alpha, and its full induction requires the presence of the transcription factor, c-FOS, This family of transcription factors also plays a role in induction of human collagenase-3, since it binds to the AP-1 site of this matrix metalloproteinase.