Scanning Electron Microscopic Features of the External and Internal Surfaces of Normal Adult Lacrimal Drainage System
Scanning Electron Microscopic Features of the External and Internal Surfaces of Normal Adult Lacrimal Drainage System
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DOI：
10.1097/iop.0000000000000489
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发表时间：
2015-09-01
影响因子：
2
通讯作者：
Naik, Milind N.
中科院分区：
文献类型：
--
作者：
Ali, Mohammad Javed;Baig, Farhana;Naik, Milind N.
目的：本研究的目的是检查的外部和内部表面的健康泪道引流systems.Methods：一个前瞻性的介入研究健康成人泪道引流系统从新鲜exentered标本上获得的超微结构特征。对于术前泪腺评估正常的与泪腺系统无关的恶性肿瘤，进行了切除术。进行了仔细和彻底的解剖，以分离从泪点到鼻泪管的整个泪道引流系统。使用扫描电子显微镜的标准协议进行分析。结果：内泪点表面表现出一个明确的和略有升高的交界处之间的管腔表面的泪点和垂直小管的开始。泪囊与鼻泪管之间有相似的连接。泪小管的瓣膜显示出宽的皱褶样粘膜表面，而泪小管的外表面显示出具有胶原附着的轮廓分明的眼轮匝肌。泪囊壁和鼻泪管壁可见密集的血管丛，其中包括宽腔动脉、节流静脉和大容量血管。结论：泪道引流系统内外表面的超微结构特征有助于更好地了解其解剖和生理。泪点-垂直小管和泪囊-鼻泪管的交界区有待进一步探索。
Aim: The aim of this study was to examine the ultrastructural features of the external and internal surfaces of healthy lacrimal drainage systems.Methods: A prospective interventional study was performed on the healthy adult lacrimal drainage systems obtained from fresh exenterated specimens. Exenteration was performed for malignancies unrelated to lacrimal system where preoperative lacrimal evaluation was normal. A careful and thorough dissection was carried out to isolate the entire lacrimal drainage system from the punctum to the nasolacrimal duct. The analysis was performed using the standard protocols of scanning electron microscopy.Results: Inner punctal surfaces showed a definite and slightly elevated junction between the luminal surfaces of punctum and beginning of the vertical canaliculus. Similar junction could be identified between the lacrimal sac and nasolacrimal duct. The valves of the canaliculi showed broad rugae-like mucosal surfaces, whereas the external surfaces of the canaliculi demonstrated well-defined orbicularis muscle with collagenous attachments. The walls of the lacrimal sac and nasolacrimal duct showed dense vascular plexus which included wide luminal arteries, throttle veins, and large capacitance vessels.Conclusions: Ultrastructural features of external and internal surfaces of lacrimal drainage system help in better understanding of its anatomy and physiology. The junctional area between the punctum-vertical canaliculus and lacrimal sac-nasolacrimal duct needs further exploration to understand their roles.