Bead-to-bead transfer of Vero cells in microcarrier culture
Bead-to-bead transfer of Vero cells in microcarrier culture
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DOI：
10.1023/a:1008079013375
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发表时间：
1999-01-01
期刊：
影响因子：
2.2
通讯作者：
Fan, OY
中科院分区：
文献类型：
--
作者：
Wang, YS;Fan, OY
细胞收获技术在微载体培养贴壁依赖性细胞的规模化中具有重要意义。传统的方法往往费时费力，并对细胞造成生理损伤。珠到珠细胞转移为规模放大过程提供了有吸引力的解决方案。通过间歇搅拌，实现了成功的细胞转移。在裸珠与汇合珠接触的地方观察到显著的细胞生长。大多数新鲜的微载体在加入培养基后4天达到接近汇合。
Cell harvesting technique is of considerable importance in the scale-up of microcarrier cultures of anchorage-dependent cells. The traditional methods are often time- and labor-consuming and cause physiological damage to the cells. Bead-to-bead cell transfer provides an attractive solution to the scale up process. By intermittent agitation, successful cell transfer was achieved. Significant cell growth was observed where bare beads contacted with confluent ones. Most of the fresh microcarriers reached near confluence four days after addition into the culture medium.