GRADIENT-TAILORED EXCITATION FOR SINGLE-QUANTUM NMR-SPECTROSCOPY OF AQUEOUS-SOLUTIONS
GRADIENT-TAILORED EXCITATION FOR SINGLE-QUANTUM NMR-SPECTROSCOPY OF AQUEOUS-SOLUTIONS
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DOI：
10.1007/bf02192855
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发表时间：
1992-11-01
影响因子：
2.7
通讯作者：
SKLENAR, V
中科院分区：
文献类型：
--
作者：
PIOTTO, M;SAUDEK, V;SKLENAR, V
描述了一种用于测量非氘化水溶液中核磁共振光谱的定制选择性激发的新方法（水门，水抑制梯度定制激发）。梯度回波序列有效地结合了一个选择性180度射频脉冲和两个场梯度脉冲，实现了高选择性和有效的抑水。该技术非常适合快速收集多维数据，因为单次扫描采集产生具有完美平坦基线的纯相NMR谱，具有最高的灵敏度。应用于快速测量2.2的二维NOE数据。双链DNA片段的mM溶液在90%的水在5℃。
A novel approach to tailored selective excitation for the measurement of NMR spectra in non-deuterated aqueous solutions (WATERGATE, WATER suppression by GrAdient-Tailored Excitation) is described. The gradient echo sequence, which effectively combines one selective 180-degrees radiofrequency pulse and two field gradient pulses, achieves highly selective and effective water suppression. This technique is ideally suited for the rapid collection of multi-dimensional data since a single-scan acquisition produces a pure phase NMR spectrum with a perfectly flat baseline, at the highest possible sensitivity. Application to the fast measurement of 2D NOE data of a 2.2. mM solution of a double-stranded DNA fragment in 90% H2O at 5-degrees-C is presented.