The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo.
The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo.
复制标题
钙颗粒蛋白B基因对宫颈鳞癌体内外生物学行为的影响
DOI：
10.2147/cmar.s153036
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Zhu X
中科院分区：
文献类型：
--
作者：
Zhang W;Chen M;Cheng H;Shen Q;Wang Y;Zhu X
目的探讨钙颗粒蛋白B基因对宫颈鳞癌生物学行为的影响。方法利用癌症基因组图谱数据库，鉴定人乳头瘤病毒（HPV）阳性和阴性宫颈癌组之间钙颗粒蛋白B基因的差异转录，并探讨钙颗粒蛋白B基因与基质金属蛋白酶（MMP）基因的关系。随后，通过宫颈癌细胞中钙颗粒蛋白B的过表达和/或低表达，研究了钙颗粒蛋白B对细胞增殖、凋亡、侵袭和迁移的作用。此外，通过构建钙颗粒蛋白B过表达或低表达的BALB/c裸鼠异种移植模型，研究钙颗粒蛋白B对宫颈癌生长的影响。结果宫颈癌中钙颗粒蛋白B基因转录与高危型HPV-16和HPV-45高度相关。此外，钙颗粒蛋白B的过度表达增加了细胞增殖、侵袭和迁移，但并没有显着影响细胞凋亡。钙颗粒蛋白 B 敲低测定也证实了这种效果。此外，我们发现在宫颈癌中钙颗粒蛋白B基因的转录与MMP15和MMP24基因呈负相关，但与MMP25基因呈正相关。此外，钙颗粒蛋白B显着促进体内宫颈癌的生长。结论 钙颗粒蛋白B可能通过调节MMPs促进宫颈鳞癌细胞增殖、迁移和侵袭。钙粒蛋白B与HPV-16/HPV-45感染对宫颈鳞状细胞癌的发生或进展是否存在协同作用尚需进一步研究。
Objective The objective of the study was to explore the role of calgranulin B gene on the biological behavior of squamous cervical cancer. Methods Differential transcription in calgranulin B gene between human papillomavirus (HPV)-positive and negative cervical cancer groups was identified, and the relationship between calgranulin B gene and matrix metalloproteinase (MMP) genes were explored using The Cancer Genome Atlas database. Subsequently, the role of calgranulin B on the cell proliferation, apoptosis, invasion and migration was investigated, through overexpression and/or underexpression of calgranulin B in cervical cancer cells. In addition, the effect of calgranulin B on the growth of the cervical cancer was studied via constructing xenograft model in BALB/c nude mice that either overexpressed or underexpressed calgranulin B. Results Calgranulin B gene transcription in cervical cancer was highly correlated with the high-risk HPV-16 and HPV-45. In addition, overexpression of calgranulin B increased cell proliferation, invasion and migration, whereas it did not significantly affect cell apoptosis. This effect was also confirmed by calgranulin B knockdown assay. Additionally, we found that the transcription of calgranulin B gene was negatively correlated with MMP15 and MMP24 genes, but positively associated with MMP25 genes in cervical cancer. Furthermore, calgranulin B significantly promoted the growth of cervical cancer in vivo. Conclusion Calgranulin B promotes cell proliferation, migration and invasion of squamous cervical cancer, possibly via regulation of MMPs. Whether there are synergistic actions between calgranulin B and HPV-16/HPV-45 infection on the squamous cervical carcinogenesis or progression need further study.