COMPLEMENT COMPONENTS C1Q, C1R/C1S, AND C1INH IN RHEUMATOID-ARTHRITIS - CORRELATION OF IN-SITU HYBRIDIZATION AND NORTHERN BLOT RESULTS WITH FUNCTION AND PROTEIN CONCENTRATION IN SYNOVIUM AND PRIMARY CELL CULTURES
COMPLEMENT COMPONENTS C1Q, C1R/C1S, AND C1INH IN RHEUMATOID-ARTHRITIS - CORRELATION OF IN-SITU HYBRIDIZATION AND NORTHERN BLOT RESULTS WITH FUNCTION AND PROTEIN CONCENTRATION IN SYNOVIUM AND PRIMARY CELL CULTURES
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DOI：
10.1002/art.1780380406
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发表时间：
1995-04-01
影响因子：
--
通讯作者：
LOOS, M
中科院分区：
文献类型：
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作者：
BREITNER, S;STORKEL, S;LOOS, M
目的：分析类风湿关节炎（RA）患者滑膜部位及表达Clq、C1q/C1s和c1 -酯酶抑制剂（C1INH）的细胞类型，并对新合成的Clq进行表征。采用Northern blotting、原位杂交、脉冲追踪等方法，对类风湿关节炎（RA）患者滑膜组织和原代细胞培养的Clq、C1r/C1s和C1INH进行分析。在原位杂交实验中，滑膜衬里细胞层被确定为Clq、Clr和C1INH的表达位点。相反，免疫组织学分析显示，Clq、Cls和C1INH蛋白存在于覆盖在滑膜衬里细胞上的薄膜中。原代细胞培养的原位杂交表明，只有巨噬细胞能够表达Clq，而成纤维细胞和星状细胞能够合成Clr。结论：滑膜对Clq和C1r/C1s以及对照蛋白C1INH的合成和分泌很重要，这支持了RA患者局部发生炎症过程的观点。
Objective, To analyze the synovial site and the cell types expressing Clq, C1q/C1s, and C1-esterase inhibitor (C1INH) and to characterize newly synthesized Clq in patients with rheumatoid arthritis (RA),Methods, Tissue and primary cell cultures of synovium from RA patients were analyzed for Clq, C1r/C1s, and C1INH by Northern blotting, in situ hybridization, and pulse-chase experiments for Clq,Results, The de novo synthesis of Clq, C1r/C1s, and C1INH in synovium and primary cell cultures was proven by Northern blot and by antigenic and functional analysis, In in situ hybridization experiments, the synovial lining cell layer was identified as the site of Clq, Clr, and C1INH expression, In contrast, immunohistologic analysis showed that Clq, Cls, and C1INH proteins were present in a thin film covering the synovial lining cells, In situ hybridization performed on primary cell cultures provided evidence that only macrophages were able to express Clq, whereas fibroblasts and stellate cells synthesized Clr,Conclusion, The synovium is important for the synthesis and secretion of Clq and C1r/C1s, as well as the control protein C1INH, which supports the idea of a locally occurring inflammatory process in RA patients.