Mutagenesis and reconstitution of middle-to-long-wave-sensitive visual pigments of New World monkeys for testing the tuning effect of residues at sites 229 and 233
Mutagenesis and reconstitution of middle-to-long-wave-sensitive visual pigments of New World monkeys for testing the tuning effect of residues at sites 229 and 233
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DOI：
10.1016/j.visres.2004.04.008
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发表时间：
2004-01-01
期刊：
影响因子：
1.8
通讯作者：
Kawamura, S
中科院分区：
文献类型：
--
作者：
Hiramatsu, C;Radlwimmer, FB;Kawamura, S
新世界猴的色觉多态性是由中长波敏感（M/LWS）视色素的等位基因变异引起的。在序列比较的基础上，等位基因之间的光谱差异已被归因于位点180、229、233、277和285处的氨基酸残基。虽然通过对大量脊椎动物M/LWS色素进行定点诱变（“三位点规则”），已经证明了位点180、277和285的显著光谱效应，但位点229和233的效应仍未得到检验。在这里，我们测量了来自三等位基因松鼠猴（Saimiri sciureus）和单等位基因猫头鹰猴（Aotus trivirgatus）的重组M/LWS色素的吸收光谱。Saimiri色素的最大吸收波长分别为532、545和558 nm，Aotus色素的最大吸收波长为539 nm，与三位法则的预测一致。我们对位点229和233的定点诱变表明，它们对λ（max）值的突变效应可以忽略不计。这些结果排除了检查外显子4的必要性，编码在位点229和233，M/LWS色素基因的色觉分型的新世界猴的残基。(C)2004 Elsevier Ltd.保留所有权利。
The colour vision polymorphism of New World monkeys results from allelic variations of the middle-to-long-wave-sensitive (M/LWS) visual pigments. On the basis of sequence comparison, spectral differences among the alleles have been ascribed to amino acid residues at sites 180, 229, 233, 277, and 285. While the significant spectral effects have been demonstrated for sites 180, 277, and 285 by site-directed mutagenesis for a large number of vertebrate M/LWS pigments (the "three-site rule"), effects at sites 229 and 233 remain untested. Here we measured absorption spectra of the reconstituted M/LWS pigments from the tri-allelic squirrel monkey (Saimiri sciureus) and the mono-allelic owl monkey (Aotus trivirgatus). The peak absorption spectra (lambda(max)) of Saimiri pigments were 532, 545, and 558 nm and that of Aotus pigment 539 nm, being consistent with the prediction from the three-site rule. Our site-directed mutagenesis for sites 229 and 233 showed that their mutational effects for lambda(max) values were negligible. These results preclude the necessity of examining exon 4, encoding the residues at sites 229 and 233, of M/LWS pigment genes for colour-vision typing of New World monkeys. (C) 2004 Elsevier Ltd. All rights reserved.