Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.).
Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.).
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DOI：
10.1093/jexbot/53.368.399
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发表时间：
2002-03
影响因子：
6.9
通讯作者：
K. Shibuya;M. Nagata;N. Tanikawa;Toshihito Yoshioka;T. Hashiba;S. Satoh
中科院分区：
文献类型：
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作者：
K. Shibuya;M. Nagata;N. Tanikawa;Toshihito Yoshioka;T. Hashiba;S. Satoh
在香石竹（Dianthus caryophyllus L.）中发现了3个乙烯受体基因DC-ERS 1、DC-ERS 2和DC-ETR 1。在此，通过北方印迹分析研究花组织中相应基因的mRNA的存在及其在花衰老期间的变化。DC-ERS 2和DC-ETR 1 mRNA在完全开放期的花瓣、子房和花柱中均有大量表达。在花瓣中，DC-ERS 2 mRNA的水平呈下降趋势的花衰老后期，而它略有增加，在整个衰老期间的风格是不变的子房。然而，DC-ETR 1 mRNA在衰老过程中的任何组织中没有或几乎没有变化。外源施加乙烯不影响花瓣中DC-ERS 2和DC-ETR 1 mRNA的水平。用1，1-二甲基-4-（苯磺酰基）氨基脲（DPSS）处理可以阻断花中乙烯的产生，但花瓣中DC-ERS 2和DC-ETR 1的mRNA水平下降。所有病例均未检测到DC-ERS 1 mRNA。这些结果表明，DC-ERS 2和DC-ETR 1是乙烯受体基因负责乙烯的感知和它们的表达受到调控的组织特异性的方式和独立的乙烯在康乃馨花衰老过程中。
Three ethylene receptor genes, DC-ERS1, DC-ERS2 and DC-ETR1, were previously identified in carnation (Dianthus caryophyllus L.). Here, the presence of mRNAs for respective genes in flower tissues and their changes during flower senescence are investigated by Northern blot analysis. DC-ERS2 and DC-ETR1 mRNAs were present in considerable amounts in petals, ovaries and styles of the flower at the full-opening stage. In the petals the level of DC-ERS2 mRNA showed a decreasing trend toward the late stage of flower senescence, whereas it increased slightly in ovaries and was unchanged in styles throughout the senescence period. However, DC-ETR1 mRNA showed no or little changes in any of the tissues during senescence. Exogenously applied ethylene did not affect the levels of DC-ERS2 and DC-ETR1 mRNAs in petals. Ethylene production in the flowers was blocked by treatment with 1,1-dimethyl-4-(phenylsulphonyl)semicarbazide (DPSS), but the mRNA levels for DC-ERS2 and DC-ETR1 decreased in the petals. DC-ERS1 mRNA was not detected in any cases. These results indicate that DC-ERS2 and DC-ETR1 are ethylene receptor genes responsible for ethylene perception and that their expression is regulated in a tissue-specific manner and independently of ethylene in carnation flowers during senescence.