ACTIVITY OF A RECOMBINANT FUSION PROTEIN BETWEEN TRANSFORMING GROWTH-FACTOR TYPE-ALPHA AND PSEUDOMONAS TOXIN
ACTIVITY OF A RECOMBINANT FUSION PROTEIN BETWEEN TRANSFORMING GROWTH-FACTOR TYPE-ALPHA AND PSEUDOMONAS TOXIN
复制标题
DOI：
10.1073/pnas.84.13.4538
复制
发表时间：
1987-07-01
影响因子：
11.1
通讯作者：
PASTAN, I
中科院分区：
文献类型：
--
作者：
CHAUDHARY, VK;FITZGERALD, DJ;PASTAN, I
转化生长因子α型基因已经融合到一个修饰的假单胞菌毒素基因，从其中的细胞识别结构域已经删除。该嵌合基因已在大肠杆菌中表达，并获得了嵌合蛋白。PE 40-TGF-α，已经被高度净化了PE40-TGF-.alpha.杀死表达表皮生长因子受体的细胞，并且对具有很少受体的细胞几乎没有活性。这种嵌合蛋白可能有助于治疗含有大量表皮生长因子受体的癌症。
The transforming growth factor type .alpha. gene has been fused to a modified Pseudomonas toxin gene from which the cell-recognition domain has been deleted. The chimeric gene has been expressed in Escherichia coli, and the chimeric protein. PE40-TGF-.alpha., has been highly purified. PE40-TGF-.alpha. kills cells expressing epidermal growth factor receptors and has little activity against cells with few receptors. This chimeric protein might be useful in treating cancers that contain high numbers of epidermal growth factor receptors.