Fez1 is layer-specifically expressed in the adult mouse neocortex
Fez1 is layer-specifically expressed in the adult mouse neocortex
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DOI：
10.1111/j.1460-9568.2004.03763.x
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发表时间：
2004-12-01
影响因子：
3.4
通讯作者：
Takumi, T
中科院分区：
文献类型：
--
作者：
Inoue, K;Terashima, T;Takumi, T
哺乳动物大脑新皮质占据大脑皮质的最大面积，并且在细胞结构上由六层（I-VI）组成。最近的分子分析已经开始揭示各种发育程序的存在，包括新皮层区域化的遗传调节。虽然越来越多的分子决定因素的发育阶段的新皮层已被确定，没有基因特异性表达的成人新皮层已被确定的日期。通过使用微阵列的全球筛选，结合系统的原位杂交，我们确定了一个锌指型转录因子，Fez 1，这是主要在小鼠成年新皮质中表达。迄今为止，新皮层中没有其他基因表现出如此高的特异性。使用双色原位杂交，我们发现，Fez 1主要表达在皮质层V和VI，而不是在γ-氨基丁酸神经元，但在锥体神经元，大脑皮层的投射神经元。免疫组织化学还显示Fez 1在新皮质的深层中表达。Fez 1将是非常宝贵的，不仅为皮质发育的分子理解，但也为了解成人新皮质的生理功能，以及在基因操作的动物和条件表达系统中使用其启动子。
The mammalian cerebral neocortex occupies the largest area of the cerebral cortex and is cytoarchitectually composed of six layers (I-VI). Recent molecular analysis has begun to reveal the existence of various developmental programs, including the genetic regulation of arealization of the neocortex. Although an increasing number of molecular determinants of the developmental stages of the neocortex have been identified, no genes specifically expressed in the adult neocortex have been identified to date. By global screening using microarrays, combined with systematic in situ hybridization, we identified a zinc-finger type transcription factor, Fez1, which is expressed predominantly in the mouse adult neocortex. No other genes in the neocortex have been shown to date to have their expression with such high specificity. Using two-color in situ hybridization, we show that Fez1 is mainly expressed in cortical layers V and VI, not in gamma-aminobutyric acid neurons but in pyramidal neurons, the projection neurons of the cerebral cortex. Immunohistochemistry also shows that Fez1 is expressed in deep layers of the neocortex. Fez1 will be invaluable not only for the molecular understanding of corticogenesis but also for understanding the physiological functions of the adult neocortex, as well as for the use of its promoter in gene-manipulated animals and in conditional expression systems.