Raman spectroscopy detects biochemical changes due to proliferation in mammalian cell cultures
Raman spectroscopy detects biochemical changes due to proliferation in mammalian cell cultures
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DOI：
10.1529/biophysj.103.038604
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发表时间：
2005-06-01
影响因子：
3.4
通讯作者：
Mourant, JR
中科院分区：
文献类型：
--
作者：
Short, KW;Carpenter, S;Mourant, JR
测量了生长的平台期（非增殖期）和指数期（增殖期）培养物的细胞和细胞核的拉曼光谱，并表明拉曼光谱可以监测由于细胞增殖引起的变化。一个简单的拟合程序开发使用的基础设置（脂质，蛋白质，DNA，RNA），以估计细胞和细胞核中的生化成分的相对量。使用生物化学组分的相对量和比率，可以检测和量化通过光谱中的振动带的视觉分析不容易明显的可再现的差异。这些差异，由于细胞增殖，可以分配到特定的生化变化。它们包括非致瘤指数细胞和细胞核的相对脂质减少和相对蛋白质和RNA增加，以及致瘤指数细胞的相对RNA增加。非致瘤指数细胞和细胞核以及致瘤指数细胞的脂质/RNA比率降低。致瘤和非致瘤指数细胞和细胞核的蛋白质/脂质比增加。最后，脂质/DNA比例降低的肿瘤指数核。这些知识对于体内快速分裂的癌细胞群体的拉曼检测将是重要的。
Raman spectra of cells and nuclei from cultures in the plateau (nonproliferating) and exponential (proliferating) phases of growth were measured and show that Raman spectroscopy can monitor changes due to cell proliferation. A simple fitting routine was developed using a basis set (lipid, protein, DNA, RNA) to estimate the relative amounts of biochemical components in cells and nuclei. Using relative amounts and ratios of biochemical components, reproducible differences can be detected and quantified that are not readily apparent by visual analysis of vibrational bands in the spectra. These differences, due to cell proliferation, can be assigned to specific biochemical changes. They include a decrease in the relative lipid and increases in the relative protein and RNA for both nontumorigenic exponential cells and nuclei, and an increase in the relative RNA for tumorigenic exponential cells. The lipid/RNA ratio decreases for nontumorigenic exponential cells and nuclei and tumorigenic exponential cells. The protein/lipid ratio increases for both tumorigenic and nontumorigenic exponential cells and nuclei. Finally, the lipid/DNA ratio decreases for tumorigenic exponential nuclei. This knowledge will be important for Raman detection of rapidly dividing populations of cancer cells in vivo.