Rice black-streaked dwarf virus-encoded P5-1 regulates the ubiquitination activity of SCF E3 ligases and inhibits jasmonate signaling to benefit its infection in rice
Rice black-streaked dwarf virus-encoded P5-1 regulates the ubiquitination activity of SCF E3 ligases and inhibits jasmonate signaling to benefit its infection in rice
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DOI：
10.1111/nph.16066
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发表时间：
2019-08-09
期刊：
影响因子：
9.4
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
He, Long;Chen, Xuan;Yang, Jian
SCF （Skp1/Cullin1/F-box）复合物是许多细胞过程的关键调节因子。病毒编码特定的因子来干扰或劫持这些复合物，并确保它们在植物中感染。控制这种干扰/劫持的分子机制目前在很大程度上是未知的。在这里，我们提出了水稻黑条纹矮病毒（RBSDV）通过干扰OsCSN5A的活性来调节水稻泛素化的新策略的证据。我们还发现RBSDV P5-1特异性影响csn介导的OsCUL1的去乙酰化，损害SCFCOI1复合物的完整性。研究表明，在转基因P5-1植株中，茉莉酸（JA）生物合成相关基因的表达不受抑制，而茉莉酸应答基因的表达则下调。更重要的是，对P5-1转基因植株施用JA并没有降低其对RBSDV感染的易感性。我们的研究结果表明，P5-1通过与OsCSN5A相互作用抑制SCF E3连接酶的泛素化活性，并阻碍CUL1的RUBylation/ deubylation，从而抑制JA反应途径，增强水稻RBSDV感染。
SCF (Skp1/Cullin1/F-box) complexes are key regulators of many cellular processes. Viruses encode specific factors to interfere with or hijack these complexes and ensure their infection in plants. The molecular mechanisms controlling this interference/hijack are currently largely unknown. Here, we present evidence of a novel strategy used by Rice black-streaked dwarf virus (RBSDV) to regulate ubiquitination in rice (Oryza sativa) by interfering in the activity of OsCSN5A. We also show that RBSDV P5-1 specifically affects CSN-mediated deRUBylation of OsCUL1, compromising the integrity of the SCFCOI1 complex. We demonstrate that the expressions of jasmonate (JA) biosynthesis-associated genes are not inhibited, whereas the expressions of JA-responsive genes are down-regulated in transgenic P5-1 plants. More importantly, application of JA to P5-1 transgenic plants did not reduce their susceptibility to RBSDV infection. center dot Our results suggest that P5-1 inhibits the ubiquitination activity of SCF E3 ligases through an interaction with OsCSN5A, and hinders the RUBylation/deRUBylation of CUL1, leading to an inhibition of the JA response pathway and an enhancement of RBSDV infection in rice.