Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment
Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment
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DOI：
10.2164/jandrol.04146
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发表时间：
2005-05-01
影响因子：
--
通讯作者：
Tesarik, J
中科院分区：
文献类型：
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作者：
Greco, E;Iacobelli, M;Tesarik, J
众所周知，精子DNA碎片化会损害男性的生育能力。先前的研究结果表明，氧化应激在这种病理状态的病因学中有一定的意义。本研究旨在探讨口服两种抗氧化剂维生素C和维生素E是否可以减少精液中DNA片段化的病理性增加的发生率。64名患有不明原因不育症且精液中DNA片段化精子比例升高(=15%)的男性被随机分为抗氧化剂治疗组(每日1g维生素C和1g维生素E，持续2个月)和安慰剂组。用末端脱氧核糖核苷酸转移酶介导的dUTP缺口末端标记法检测治疗前后精子DNA断裂情况。抗氧化剂组和安慰剂组在治疗前后基本精子参数没有发现差异。然而，抗氧化剂治疗组治疗后DNA碎裂精子百分率(9.1+/-7.2)较治疗前(22.1+/-7.7)显著降低(P<；.001)。安慰剂组治疗前后精子DNA断裂发生率无差异。这些数据表明，口服抗氧化剂可以在相对较短的时间内有效地治疗精子DNA损伤。
Sperm DNA fragmentation is known to compromise male fertility. Previous findings have suggested the implication of oxidative stress in the etiology of this pathological condition. The present study was conducted to find out if the pathologically increased incidence of DNA fragmentation in ejaculated spermatozoa can be reduced by oral treatment with two antioxidants, vitamins C and E. Sixty-four men with unexplained infertility and an elevated (>= 15%) percentage of DNA-fragmented spermatozoa in the ejaculate were randomized between an antioxidant treatment (1 g vitamin C and 1 g vitamin E daily for 2 months) group and a placebo group. Sperm DNA fragmentation was evaluated by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling assay before and after treatment. No differences in basic sperm parameters were found between the antioxidant treatment and the placebo group before or after treatment. However, the percentage of DNA-fragmented spermatozoa was markedly reduced (P < .001) in the antioxidant treatment group after the treatment (9.1 +/- 7.2) as compared with the pretreatment values (22.1 +/- 7.7). No difference in the pretreatment and posttreatment incidence of sperm DNA fragmentation was observed in the placebo group. These data show that sperm DNA damage can be efficiently treated with oral antioxidants administered during a relatively short time period.