LUMINOL CHEMI-LUMINESCENCE - CHEMISTRY, EXCITATION, EMITTER
LUMINOL CHEMI-LUMINESCENCE - CHEMISTRY, EXCITATION, EMITTER
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DOI：
10.1002/bio.1170050111
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
ERIKSEN, TE
中科院分区：
文献类型：
--
作者：
MERENYI, G;LIND, J;ERIKSEN, TE
鲁米诺化学发光的机理是羰基化合物亲核加成的一种特殊情况。关键中间体α-羟基过氧化氢的分解产生了与酰基双氮烯邻位的过氧酸。过氧酸在分子内加成后，从氮气中排出的能量被用来形成抗芳香族内过氧物。沿着O，O键的断裂使随后生成的邻苯二甲酸盐的很大一部分处于激发状态。该发射体是一种单质子化的邻苯二甲酸盐，不能被光激发。暗反应是α-羟基过氧化氢的协同分解，生成基态邻苯二甲酸酯。
The mechanism of luminol chemiluminescence is a special case of nucleophilic addition to carbonyl compounds. The breakdown of the key intermediate, an alpha hydroxy hydroperoxide, produces a peracid ortho to an acyl diazene group. After intramolecular addition of the peracid, the energy from nitrogen expulsion is utilized in the formation of an anti‐aromatic endoperoxide. Rupture along the O,O bond leaves a substantial part of the ensuing phthalate in its excited state. The emitter is shown to be a mono‐protonated phthalate unaccessible by photoexcitation. The dark reaction is a concerted decomposion of the alpha hydroxy hydroperodixe to yield ground‐state phthalate.