Electrodeposited iridium oxide pH electrode for measurement of extracellular myocardial acidosis during acute ischemia
Electrodeposited iridium oxide pH electrode for measurement of extracellular myocardial acidosis during acute ischemia
复制标题
DOI：
10.1021/ac980608e
复制
发表时间：
1998-12-01
影响因子：
7.4
通讯作者：
Cascio, WE
中科院分区：
文献类型：
--
作者：
Marzouk, SAM;Ufer, S;Cascio, WE
在本文中，固态pH电极的制造，表征和生理应用进行了描述。pH敏感层基于阳极电沉积的氧化铱膜（AEIROF），在柔性Kapton膜或铂丝上制造的溅射铂电极（1 mm直径）用作平面或圆柱形支撑物。每个电极部位都涂有Nafion，以减弱阴离子氧化还原物质的干扰，并在体内测量过程中保护电极表面。AEIROF的性能首次作为pH电极进行了评估，并证明其具有轻微的超能斯特响应，对于线和平面溅射铂电极的斜率均为-63.5 +/- 2.2 mV/pH单位。在pH 2-10范围内，电极的pH响应呈线性，电极的工作寿命至少为1个月，精度约为0.02pH单位，响应时间快。电极对Na+、K+、Li+、NH 4+、Ca ~（2+）、Mg ~（2+）、溶解氧、乳酸、抗坏血酸和马黛茶等生理学上重要的物质的灵敏度很低。第一份报告的细胞外pH值，K+和乳酸盐同时测量在无血流缺血期间使用AEIROF pH电极和先前描述的K+和乳酸盐电极。
In the present paper, fabrication, characterization, and physiological applications of a solid-state pH electrode are described. The pH sensing layer was based on an anodic electrodeposited iridium oxide film (AEIROF), Sputtered platinum electrodes (1 mm diameter) fabricated on flexible Kapton films or platinum wires were used as planar or cylindrical supports. Each electrode site was coated with Nafion to attenuate the interference of anionic redox species and to protect the electrode surface during in vivo measurements. Performance of the AEIROF was evaluated, for the first time, as a pH electrode and proved to have a slightly super-Nernstian response with slope of -63.5 +/- 2.2 mV/pH unit for both wire and planar sputtered platinum electrodes. Linear pH responses were obtained in the pH range 2-10, The electrodes have a working lifetime of at least 1 month with accuracy of about 0.02 pH unit and fast response time. The electrodes showed very low sensitivities for different species, such as Na+, K+, Li+, NH4+, Ca2+, Mg2+, dissolved oxygen, lactate, ascorbate, and mate, which are important for physiological applications, The electrodes were applied in extracellular pH measurements during brief regional ischemia in a swine heart and no-flow ischemia in an isolated rabbit papillary muscle. A first report on extracellular pH, K+, and lactate simultaneous measurements during no-flow ischemia using the AEIROF pH electrode and the previously described K+ and lactate electrodes is presented as well.