Single-Cell RNA Sequencing with Drop-Seq
Single-Cell RNA Sequencing with Drop-Seq
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DOI：
10.1007/978-1-4939-9240-9_6
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Raddi, Gianmarco
中科院分区：
文献类型：
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作者：
Bageritz, Josephine;Raddi, Gianmarco
Drop-Seq 是一种低成本、高通量平台，可通过将数千个细胞封装到单个液滴中来分析它们。带有独特条形码的 mRNA 捕获微粒和细胞通过微流体装置共同限制在液滴内，在那里它们进行细胞裂解和 RNA 杂交。在打破液滴并汇集杂交颗粒后，在单个反应中进行逆转录、PCR 和测序可以从数千个单细胞转录组中生成数据，同时保留有关每个转录本的细胞起源的信息。
Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate data from thousands of single-cell transcriptomes while maintaining information on the cellular origin of each transcript.