Expression pattern of VEGFR-2 (Quek1) during quail development
Expression pattern of VEGFR-2 (Quek1) during quail development
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DOI：
10.1007/s00429-004-0396-z
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发表时间：
2004-06-01
期刊：
影响因子：
--
通讯作者：
Huang, R
中科院分区：
文献类型：
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作者：
Nimmagadda, S;Loganathan, PG;Huang, R
血液和淋巴血管系统的生长和维持在很大程度上是由血管内皮生长因子（VEGF）家族成员通过在成血管细胞中表达的酪氨酸激酶受体（VEGFRs）控制的。本研究采用全囊原位杂交技术分析了鹌鹑发育过程中Quek1 （VEGFR-2）的表达模式。在胚胎发生早期，在胚层尾端和原始条纹以及头部近轴中胚层中检测到Quek1的表达。在一些体中，从hh - 9期开始，在形成和新近形成的一些体的背外侧区域都观察到表达。在体细胞成熟过程中，这种表达持续存在于体细胞室的外侧部分、皮细胞组和核细胞组。此外，在成熟的体体中，在靠近神经管的硬核组内侧观察到第二个表达域。该表达域向内侧和背侧延伸，并在后期包围神经管。在脊索中，从hh - 23期开始观察到表达。在肢芽中，hh - 17期在间质中开始表达。在器官发生过程中，在咽弓和食管、气管、胃、肺、肝、心、肠的原纤维中均有表达。从第7天开始，在羽芽中也可以看到表达。我们的研究结果证实了中胚层的血管生成潜力，并表明表达VEGFR-2的细胞代表了造血和血管生成谱系的多个中胚层前体。
The growth and maintenance of the blood and lymphatic vascular systems is to a large extent controlled by members of the vascular endothelial growth factor (VEGF) family via the tyrosine kinase receptors (VEGFRs) expressed in angioblastic cells. Here, we analyzed the Quek1 (VEGFR-2) expression pattern by whole mount in situ hybridization during quail development. During early embryogenesis, Quek1 expression was detected at the caudal end of the blastoderm and primitive streak and in the head paraxial mesoderm. In somites, expression was observed from HH-stage 9 onwards in the dorsolateral region of both the forming and recently formed somites. During somite maturation, expression persists in the lateral portion of the somitic compartments, the dermomyotome and the sclerotome. Additionally, a second expression domain in the maturing somite was observed in the medial part of the sclerotome adjacent to the neural tube. This expression domain extended medially and dorsally and surrounded the neural tube during later stages. In the notochord, expression was observed from HH-stage 23 onwards. In the limb bud, expression was initiated in the mesenchyme at HH-stage 17. During organogenesis, expression was detected in the pharyngeal arches and in the anlagen of the esophagus, trachea, stomach, lungs, liver, heart and gut. Expression was also seen in feather buds from day 7 onwards. Our results confirm the angiogenic potential of the mesoderm and suggest that VEGFR-2 expressing cells represent multiple pools of mesodermal precursors of the hematopoietic and angiopoietic lineages.