Defining hippocampal area CA2 in the fox (Vulpes vulpes) brain.
Defining hippocampal area CA2 in the fox (Vulpes vulpes) brain.
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定义狐狸 (Vulpes vulpes) 大脑中的海马区 CA2。
DOI：
10.1002/hipo.23546
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发表时间：
2023
期刊：
影响因子：
3.5
通讯作者：
Kukekova,AnnaV
中科院分区：
文献类型：
--
作者：
Dudek,SerenaM;Phoenix,AshleyN;Scappini,Erica;Shepeleva,DaryaV;Herbeck,YuryE;Trut,LyudmilaN;Farris,Shannon;Kukekova,AnnaV
自1959年以来，俄罗斯农场狐狸的研究已经培育出了要么驯服，要么最近，侵略性的狐狸，科学家们利用它们来深入了解与这些行为特征相关的大脑结构。在小鼠中，海马CA2区已经成为社会攻击的重要调节器之一，因此，为了最终确定我们是否可以识别驯服和攻击性狐狸之间的CA2差异，我们首先试图识别狐狸（Vulpes vulpes）中的CA2。由于在猫、狗或猪等物种中没有明确定义的CA2区域，因此根本不清楚是否可以在狐狸中鉴定出CA2。在这项研究中，我们垂直于海马长轴切割雄性和雌性红狐的颞叶切片，并用大鼠和小鼠组织中常用的CA 2锥体细胞标记物对其进行染色。我们观察到，抗浦肯野细胞蛋白4的抗体最好地染色的区域中的锥体细胞跨越苔藓纤维的末端和缺乏苔藓纤维的锥体细胞的开始，类似于在大鼠和小鼠中看到的模式。我们的研究结果表明，狐狸确实有一个“分子定义”的CA2，而且，他们还表明，其他食肉动物，如狗和猫，也可能有。在这种情况下，这些狐狸可能在未来的研究中有用，因为它与攻击性有关。
Since 1959, the Russian Farm‐Fox study has bred foxes to be either tame or, more recently, aggressive, and scientists have used them to gain insight into the brain structures associated with these behavioral features. In mice, hippocampal area CA2 has emerged as one of the essential regulators of social aggression, and so to eventually determine whether we could identify differences in CA2 between tame and aggressive foxes, we first sought to identify CA2 in foxes (Vulpes vulpes). As no clearly defined area of CA2 has been described in species such as cats, dogs, or pigs, it was not at all clear whether CA2 could be identified in foxes. In this study, we cut sections of temporal lobes from male and female red foxes, perpendicular to the long axis of the hippocampus, and stained them with markers of CA2 pyramidal cells commonly used in tissue from rats and mice. We observed that antibodies against Purkinje cell protein 4 best stained the pyramidal cells in the area spanning the end of the mossy fibers and the beginning of the pyramidal cells lacking mossy fibers, resembling the pattern seen in rats and mice. Our findings indicate that foxes do have a “molecularly defined” CA2, and further, they suggest that other carnivores like dogs and cats might as well. With this being the case, these foxes could be useful in future studies looking at CA2 as it relates to aggression.