Increase of isoflavones in soybean callus by Agrobacterium-mediated transformation
Increase of isoflavones in soybean callus by Agrobacterium-mediated transformation
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DOI：
10.1007/s11816-010-0143-2
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发表时间：
2010-07
影响因子：
2.4
通讯作者：
N. Jiang;Eun-Hee Jeon;J. Pak;T. Ha;I. Baek;W. Jung;Jai‐Heon Lee;D. Kim;Hong-Kyu Choi;
中科院分区：
文献类型：
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作者：
N. Jiang;Eun-Hee Jeon;J. Pak;T. Ha;I. Baek;W. Jung;Jai‐Heon Lee;D. Kim;Hong-Kyu Choi;
植物次生代谢产物因其在人类医学和营养中的重要作用而一直是研究的热点。我们使用农杆菌介导的转化方法将异黄酮合酶（IFS）基因转移到大豆[Glycine max(L.) Merr.]中，试图产生产生增加水平的次生代谢物异黄酮的转化大豆植物。尽管由于潮霉素选择不明确，生产转基因植物的试验失败了，但还是获得了转化的愈伤组织细胞系。三个测试品种的愈伤组织诱导率和程度相似，但光照对愈伤组织形成频率有正向影响，导致愈伤组织诱导率为 Kwangan 74%、Sojin 67% 和 Duyou 73%。在选择培养基上进行七至八次继代培养后，通过高效液相色谱分析转化愈伤组织系的异黄酮含量。转化的愈伤组织细胞系中异黄酮的总量比对照愈伤组织或种子中的异黄酮总量高三至六倍。鉴于异黄酮对人类健康有许多积极影响，我们有可能通过替代细胞培养系统将我们的转化愈伤组织系用于工业化，以生产高浓度的异黄酮。
Plant secondary metabolites have always been a focus of study due to their important roles in human medicine and nutrition. We transferred the isoflavone synthase (IFS) gene into soybean [Glycine max(L.) Merr.] using theAgrobacterium-mediated transformation method in an attempt to produce transformed soybean plants which produced increased levels of the secondary metabolite, isoflavone. Although the trial to produce transgenic plant failed due to unestablished hygromycin selection, transformed callus cell lines were obtained. The induction rate and degree of callus were similar among the three cultivars tested, but light illumination positively influenced the frequency of callus formation, resulting in a callus induction rate of 74% for Kwangan, 67% for Sojin, and 73% for Duyou. Following seven to eight subcultures on selection media, the isoflavone content of the transformed callus lines were analyzed by high-performance liquid chromatography. The total amount of isoflavone in the transformed callus cell lines was three- to sixfold higher than that in control callus or seeds. Given the many positive effects of isoflavone on human health, it may be possible to adapt our transformed callus lines for industrialization through an alternative cell culture system to produce high concentrations of isoflavones.