Quasi-Confocal, Multichannel Parallel Scan Hyperspectral Fluorescence Imaging Method Optimized for Analysis of Multicolor Microarrays
Quasi-Confocal, Multichannel Parallel Scan Hyperspectral Fluorescence Imaging Method Optimized for Analysis of Multicolor Microarrays
复制标题
针对多色微阵列分析而优化的准共焦、多通道并行扫描高光谱荧光成像方法
DOI：
10.1021/ac101629x
复制
发表时间：
2010-09-15
影响因子：
7.4
通讯作者：
He, Yonghong
中科院分区：
文献类型：
--
作者：
Liu, Zhiyi;Ma, Suihua;He, Yonghong
自20世纪末以来，微阵列技术在生物医学研究中以其高通量的并行检测能力引起了人们的极大兴趣。已经报道了许多用于微阵列检测的仪器。在本文中，我们开发了一个准共焦，多通道并行扫描高光谱荧光成像系统的微阵列研究。高光谱成像记录成像区域内每个体素的整个发射光谱，这与仅记录基于滤光片的扫描仪的荧光强度形成对比。当与数据分析相结合时，记录的光谱信息允许定量识别多种光谱重叠的荧光染料的贡献，并消除不需要的伪影。该系统改进了一个专门设计的，高性能的光谱仪，可以提供0.2 nm的光谱分辨率和操作的空间分辨率范围从2到30 μ m。我们通过阅读用于鉴定细菌的阵列来展示该系统的应用。
The microarray technique, which can provide parallel detection with high throughput in biomedical research, has generated considerable interest since the end of the 20(th) century. A number of instruments have been reported for microarray detection. In this paper, we have developed a quasi-confocal, multichannel parallel scan hyperspectral fluorescence imaging system for multicolor microarray research. Hyperspectral imaging records the entire emission spectrum for every voxel within the imaged area in contrast to recording only fluorescence intensities of filter-based scanners. When coupled with data analysis, the recorded spectral information allows for quantitative identification of the contributions of multiple, spectrally overlapping fluorescent dyes and elimination of unwanted artifacts. This system is improved with a specifically designed, high performance spectrometer which can offer a spectral resolution of 0.2 nm and operates with spatial resolutions ranging from 2 to 30 mu m. We demonstrate the application of the system by reading out arrays for identification of bacteria.