Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust
Molecular Characterization of a New Wheat-Thinopyrum intermedium Translocation Line with Resistance to Powdery Mildew and Stripe Rust
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DOI：
10.3390/ijms16012162
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发表时间：
2015-01
期刊：
影响因子：
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通讯作者：
H. Zhan;Xiaojun Zhang;Guangrong Li;Zhihui Pan;J. Hu;Xin Li;Linyi Qiao;J. Jia;Huijuan Guo
中科院分区：
文献类型：
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作者：
H. Zhan;Xiaojun Zhang;Guangrong Li;Zhihui Pan;J. Hu;Xin Li;Linyi Qiao;J. Jia;Huijuan Guo
从小麦与偃麦草杂交后代中选育出一个新的小麦-偃麦草易位系CH 13 -21。中间型部分双倍体TAI 7047和小麦品系绵阳11。采用基因组原位杂交（GISH）、多色GISH（mc-GISH）、多色荧光原位杂交（mc-FISH）和染色体特异性分子标记对CH 13 -21进行了鉴定。CH 13 -21对条锈病和白粉病的抗性表现出新的遗传特性。染色体计数分析表明，CH 13 -21有42条染色体，减数分裂中期Ⅰ具有正常的二价配对。GISH由Th探测。中间型基因组DNA分析表明，CH 13 -21含有一对小麦-Th。中间易位染色体。用pSc119.2和pAs 1作探针的连续mc-FISH分析清楚地表明，在易位染色体中，CH 13 -21的染色体臂6 BS被偃麦草染色质所取代。分子标记分析进一步证实了引入的Th. CH 13 -21的中间染色质属于同源异型群6染色体长臂。因此，CH 13 -21是一个新的T6 BS. 6Ai#1L补偿罗伯逊易位系。结果表明，CH 13 -21是一种新的小麦抗病遗传资源。
A new wheat-Thinopyrum translocation line CH13-21 was selected from the progenies derived from a cross between wheat-Th. intermedium partial amphiploid TAI7047 and wheat line Mianyang11. CH13-21 was characterized by using genomic in situ hybridization (GISH), multicolor-GISH (mc-GISH), multicolor-fluorescence in situ hybridization (mc-FISH) and chromosome-specific molecular markers. When inoculated with stripe rust and powdery mildew isolates, CH13-21 displayed novel resistance to powdery mildew and stripe rust which inherited from its Thinopyrum parent. The chromosomal counting analyses indicated that CH13-21 has 42 chromosomes, with normal bivalent pairing at metaphase I of meiosis. GISH probed by Th. intermedium genomic DNA showed that CH13-21 contained a pair of wheat-Th. intermedium translocated chromosomes. Sequential mc-FISH analyses probed by pSc119.2 and pAs1 clearly revealed that chromosome arm 6BS of CH13-21 was replaced by Thinopyrum chromatin in the translocation chromosome. The molecular markers analysis further confirmed that the introduced Th. intermedium chromatin in CH13-21 belonged to the long arm of homoeologous group 6 chromosome. Therefore, CH13-21 was a new T6BS.6Ai#1L compensating Robertsonian translocation line. It concludes that CH13-21 is a new genetic resource for wheat breeding programs providing novel variation for disease resistances.