Analysis of digoxin concentrations in serum by fluorescence polarization immunoassay: an evaluation.
Analysis of digoxin concentrations in serum by fluorescence polarization immunoassay: an evaluation.
复制标题
通过荧光偏振免疫测定法分析血清中地高辛浓度：评估。
DOI：
10.1093/jat/8.3.138
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发表时间：
1984
影响因子：
2.5
通讯作者：
K. Opheim
中科院分区：
文献类型：
--
作者：
L. Ferreri;V. Raisys;K. Opheim
评价雅培TDX荧光偏振免疫法测定地高辛血药浓度。在0.64~3.75 ng/mL浓度范围内，批内精密度(CV)小于4%。批间精密度分别为0.75 ng/m L、1.50 ng/m L、3.48 ng/m L，变异系数分别为14.5%、5.7%、4.9%。对0.2 ng/mL地高辛敏感。用放射免疫分析法(RIA)测定86例患者的TDX，相关系数r=0.94，斜率=0.93，截距=0.11，Sy/x=0.19。浓度分别为0.97和4.50 ng/m L时，平均回收率为98%。没有观察到明显的脂血症、黄疸或溶血的干扰。螺内酯与抗体无交叉反应，而洋地黄毒素表现出显著的交叉反应。与RIA程序相比，TDX检测更快速、更可靠，在这种临床情况下，更具成本效益。
The Abbott TDx fluorescence polarization immunoassay was evaluated for the determination of serum digoxin concentrations. Within-assay precision was less than 4% coefficient of variation (CV) for concentrations ranging from 0.64 to 3.75 ng/mL. Between-assay precision was 14.5% CV at 0.75 ng/mL, 5.7% CV at 1.50 ng/mL, and 4.9% CV at 3.48 ng/mL. Sensitivity to 0.2 ng/mL digoxin was confirmed. Correlation of 86 patient specimens assayed by radioimmunoassay (RIA) with the TDx showed the following: correlation coefficient r = 0.94, slope = 0.93, intercept = 0.11, and Sy/x = 0.19. Recovery from serum at concentrations of 0.97 ng/mL and 4.50 ng/mL averaged 98%. No significant interference from lipemia, icteria, or hemolysis was observed. Spironolactone showed no cross-reactivity with the antibody, while digitoxin exhibited significant cross-reactivity. Compared to the RIA procedure, the TDx assay was more rapid, reliable and, in this clinical situation, more cost effective.