THE IRIS DIAPHRAGM MODEL OF CENTRIOLE AND BASAL BODY FORMATION
THE IRIS DIAPHRAGM MODEL OF CENTRIOLE AND BASAL BODY FORMATION
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DOI：
10.1002/cm.970170307
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发表时间：
1990-01-01
影响因子：
--
通讯作者：
ALBRECHTBUEHLER, G
中科院分区：
文献类型：
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作者：
ALBRECHTBUEHLER, G
本文认为，中心粒和基体微管骨架的形成和结构可以从以下简单的几何原理推导出来。9个微管起始位点的闭环限定了（1）用于包装18个另外的微管起始位点的模板，和（2）9个刚性臂的形状。当每个臂围绕位于初始环上的四个起始位点之外的点旋转时，阵列以类似于虹膜隔膜的开口的方式展开。因此，微管三联体叶片的弯曲形状与中心粒或基体的倾斜叶片的顺时针旋转方向一起出现。中心粒（基体）的最终直径会自我调整。此外，三重叶片的螺距，中心粒和基体的锥度和叶片向远端倾斜的变化可以导出。此外，该模型指出了一种复制前中心粒（前基体）的方法。通过拟合3T3细胞中心粒的电子显微镜横截面来验证该假设，以模型预测的几何形状。
This paper suggests that the formation and structure of the microtubular skeleton of centrioles and basal bodies can be derived from the following simple geometric principle. A closed ring of nine microtubular initiation sites defines (1) a template for the packing of 18 additional microtubular initiation sites, and (2) the shape of nine rigid arms. Upon swivelling of each arm around a point located four initiation sites away on the initial ring, the array unfolds in a manner similar to the opening of an iris diaphragm. As a consequence, the curved shape of the microtubular triplet blades arises together with the clockwise rotational sense of the slanted blades of the centriole or basal body. The final diameter of the centriole (basal body) self-adjusts. Furthermore, the pitch of the triplet blades, the taper of centrioles and basal bodies and the change of slant of the blades towards the distal end can be derived. In addition, the model points to a method of replication of pro-centrioles (pro-basal bodies). The hypothesis was tested by the fitting of electron microscopical cross sections of centrioles of 3T3 cells to the geometric shapes predicted by the model.