ENERGY-COST OF WHOLE-BODY PROTEIN-SYNTHESIS MEASURED INVIVO IN CHICKS
ENERGY-COST OF WHOLE-BODY PROTEIN-SYNTHESIS MEASURED INVIVO IN CHICKS
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DOI：
10.1016/0300-9629(88)90962-0
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发表时间：
1988-01-01
影响因子：
2.3
通讯作者：
MURAMATSU, T
中科院分区：
文献类型：
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作者：
AOYAGI, Y;TASAKI, I;MURAMATSU, T
1. 通过比较注射蛋白质合成抑制剂环己亚胺后雏鸡体内蛋白质合成和产热的变化，测定全身蛋白质合成的能量成本。2. 静脉注射环己亚胺后，苯丙氨酸进入全身蛋白组分的作用被迅速抑制，且效果持续至少3小时。3. 注射环己亚胺后，全身蛋白质合成和总产热量均显著降低。4. 计算出全身蛋白质合成的能量成本为5.35 kJ / g蛋白质合成，因此在摩尔基础上，每个肽键合成需要7.52个atp。
1. Energy cost of whole-body protein synthesis was measured in vivo in chicks by comparing the changes in protein synthesis and heat production after the administration of cycloheximide, an inhibitor of protein synthesis. 2. Incorporation of phenylalanine into whole-body protein fraction was promptly inhibited after the intravenous injection of cycloheximide, and the effect was sustained for at least 3 hr. 3. Both whole-body protein synthesis and total heat production were significantly reduced by the cycloheximide administration. 4. The energy cost of whole-body protein synthesis was calculated to be 5.35 kJ per g protein synthesis, and hence on a molar basis 7.52 ATPs are required per peptide bond synthesis.